Fabrication and Validation of an Organ-on-chip System with Integrated Electrodes to Directly Quantify Transendothelial Electrical Resistance

Marinke W. van der Helm; Mathieu Odijk; Jean-Philippe Frimat; Andries D. van der Meer; Jan C.T. Eijkel; Albert van den Berg; Loes I. Segerink

doi:10.3791/56334

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Fabrication and Validation of an Organ-on-chip System with Integrated Electrodes to Directly Quantify Transendothelial Electrical Resistance

JoVE Journal
Bioinżynieria

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JoVE Journal Bioinżynieria

Fabrication and Validation of an Organ-on-chip System with Integrated Electrodes to Directly Quantify Transendothelial Electrical Resistance

16,122 Views

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10:51 min

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September 26, 2017

DOI:

10.3791/56334-v

DOI:

10.3791/56334-v

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10:51 min

September 26, 2017

•

15 Views

Marinke W. van der Helm¹, Mathieu Odijk¹, Jean-Philippe Frimat^1,2, Andries D. van der Meer³, Jan C.T. Eijkel¹, Albert van den Berg¹, Loes I. Segerink¹

¹BIOS Lab on a Chip group, MIRA Institute for Biomedical Technology and Technical Medicine, MESA+ Institute for Nanotechnology and Max Planck Center for Complex Fluid Dynamics,University of Twente, ²Microsystems,Eindhoven University of Technology, ³Applied Stem Cell Technologies, MIRA Institute for Biomedical Technology and Technical Medicine,University of Twente

Transkrypcja

Podsumowanie

This publication describes the fabrication of an organ-on-chip device with integrated electrodes for direct quantification of transendothelial electrical resistance (TEER). For validation, the blood-brain barrier was mimicked inside this microfluidic device and its barrier function was monitored. The presented methods for electrode integration and direct TEER quantification are generally applicable.