Quantitative Whole-mount Immunofluorescence Analysis of Cardiac Progenitor Populations in Mouse Embryos

Evan Bardot; Nikos Tzavaras; Deanna L. Benson; Nicole C. Dubois

doi:10.3791/56446

Dziennik

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Biologia Rozwoju

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Quantitative Whole-mount Immunofluorescence Analysis of Cardiac Progenitor Populations in Mouse Embryos

JoVE Journal
Biologia Rozwoju

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JoVE Journal Biologia Rozwoju

Quantitative Whole-mount Immunofluorescence Analysis of Cardiac Progenitor Populations in Mouse Embryos

9,544 Views

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09:42 min

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October 12, 2017

DOI:

10.3791/56446-v

DOI:

10.3791/56446-v

•

09:42 min

October 12, 2017

•

3 Views

Evan Bardot^1,2,3, Nikos Tzavaras⁴, Deanna L. Benson^5,6, Nicole C. Dubois^1,2,3

¹Cell, Developmental, and Regenerative Biology Department,Icahn School of Medicine at Mount Sinai, ²Mindich Child Health and Development Institute,Icahn School of Medicine at Mount Sinai, ³Black Family Stem Cell Institute,Icahn School of Medicine at Mount Sinai, ⁴Microscopy Core,Icahn School of Medicine at Mount Sinai, ⁵Department of Neuroscience,Icahn School of Medicine at Mount Sinai, ⁶Friedman Brain Institute,Icahn School of Medicine at Mount Sinai

Transkrypcja

Podsumowanie

Here, we describe a protocol for whole mount immunofluorescence and image-based quantitative volumetric analysis of early stage mouse embryos. We present this technique as a powerful approach to qualitatively and quantitatively assess cardiac structures during development, and propose that it may be widely adaptable to other organ systems.