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February 26, 2018
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Oogenesis in mammals is known to be error-prone, particularly due to chromosome missegregation. This manuscript describes chromatin spread preparation methods for mouse prophase, metaphase I and II-staged oocytes. These fundamental techniques allow for the study of chromatin-bound proteins and chromosome morphology throughout mammalian oogenesis.
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Hwang, G. H., Hopkins, J. L., Jordan, P. W. Chromatin Spread Preparations for the Analysis of Mouse Oocyte Progression from Prophase to Metaphase II. J. Vis. Exp. (132), e56736, doi:10.3791/56736 (2018).
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