Systematic Assessment of Well-Being in Mice for Procedures Using General Anesthesia

Katharina Hohlbaum; Bettina Bert; Silke Dietze; Rupert Palme; Heidrun Fink; Christa Th&#246;ne-Reineke

doi:10.3791/57046

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Systematic Assessment of Well-Being in Mice for Procedures Using General Anesthesia

JoVE Journal
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JoVE Journal Zachowanie

Systematic Assessment of Well-Being in Mice for Procedures Using General Anesthesia

12,093 Views

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06:50 min

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March 20, 2018

DOI:

10.3791/57046-v

DOI:

10.3791/57046-v

•

06:50 min

March 20, 2018

•

12 Views

Katharina Hohlbaum^1,2, Bettina Bert^2,3, Silke Dietze², Rupert Palme⁴, Heidrun Fink², Christa Thöne-Reineke¹

¹Institute of Animal Welfare, Animal Behavior and Laboratory Animal Science, Department of Veterinary Medicine,Freie Universität Berlin, ²Institute of Pharmacology and Toxicology, Department of Veterinary Medicine,Freie Universität Berlin, ³German Federal Institute for Risk Assessment (BfR), ⁴Unit of Physiology, Pathophysiology and Experimental Endocrinology, Department of Biomedical Sciences,University of Veterinary Medicine

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Podsumowanie

We developed a protocol to assess well-being in mice during procedures using general anesthesia. A series of behavioral parameters indicating levels of well-being as well as glucocorticoid metabolites were analyzed. The protocol can serve as a general aid to estimate the degree of severity in a scientific, animal-centered manner.