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August 30, 2019
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This islet isolation protocol described a novel route of collagenase injection to digest the exocrine tissue and a simplified gradient procedure to purify the islets from mice. It involves enzymatic digestion, gradient separation/purification, and islet hand-picking. Successful isolation can yield 250–350 high quality and fully functional islets per mouse.
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Villarreal, D., Pradhan, G., Wu, C., Allred, C. D., Guo, S., Sun, Y. A Simple High Efficiency Protocol for Pancreatic Islet Isolation from Mice. J. Vis. Exp. (150), e57048, doi:10.3791/57048 (2019).
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