Electrochemical Detection of Deuterium Kinetic Isotope Effect on Extracellular Electron Transport in <em>Shewanella oneidensis</em> MR-1

Yoshihide Tokunou; Kazuhito Hashimoto; Akihiro Okamoto

doi:10.3791/57584

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Electrochemical Detection of Deuterium Kinetic Isotope Effect on Extracellular Electron Transport in Shewanella oneidensis MR-1

Electrochemical Detection of Deuterium Kinetic Isotope Effect on Extracellular Electron Transport in <em>Shewanella oneidensis</em> MR-1

JoVE Journal
Biochemia

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JoVE Journal Biochemia

Electrochemical Detection of Deuterium Kinetic Isotope Effect on Extracellular Electron Transport in Shewanella oneidensis MR-1

Electrochemical Detection of Deuterium Kinetic Isotope Effect on Extracellular Electron Transport in Shewanella oneidensis MR-1

9,908 Views

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09:00 min

•

April 16, 2018

DOI:

10.3791/57584-v

DOI:

10.3791/57584-v

•

09:00 min

April 16, 2018

•

2 Views

Yoshihide Tokunou¹, Kazuhito Hashimoto², Akihiro Okamoto²

¹Department of Applied Chemistry,University of Tokyo, ²Global Research Center for Environment and Energy based on Nanomaterials Science,National Institute for Materials Science

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Podsumowanie

Here we present a protocol of whole-cell electrochemical experiments to study the contribution of proton transport to the rate of extracellular electron transport via the outer-membrane cytochromes complex in Shewanella oneidensis MR-1.

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Deuterium Kinetic Isotope Effect Extracellular Electron Transport Shewanella Oneidensis MR-1 Electrochemical Detection Proton Transport C-type Cytochromes Three-electrode Electrochemical Reactor ITO Substrate Anaerobic Conditions Lactate Yeast Extract

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