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April 19, 2018
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We present an automated method for three-dimensional reconstruction of the Caenorhabditis elegans germline. Our method determines the number and position of each nucleus within the germline and analyses germline protein distribution and cytoskeletal structure.
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Gopal, S., Pocock, R. Computational Analysis of the Caenorhabditis elegans Germline to Study the Distribution of Nuclei, Proteins, and the Cytoskeleton. J. Vis. Exp. (134), e57702, doi:10.3791/57702 (2018).
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