The Replica Set Method: A High-throughput Approach to Quantitatively Measure <em>Caenorhabditis elegans</em> Lifespan

Adam B. Cornwell; Jesse R. Llop; Peter Salzman; Juilee Thakar; Andrew V. Samuelson

doi:10.3791/57819

Dziennik

/

Genetyka

/

The Replica Set Method: A High-throughput Approach to Quantitatively Measure Caenorhabditis elegans Lifespan

The Replica Set Method: A High-throughput Approach to Quantitatively Measure <em>Caenorhabditis elegans</em> Lifespan

JoVE Journal
Genetyka

Aby wyświetlić tę treść, wymagana jest subskrypcja JoVE. Zaloguj się lub rozpocznij bezpłatny okres próbny.

JoVE Journal Genetyka

The Replica Set Method: A High-throughput Approach to Quantitatively Measure Caenorhabditis elegans Lifespan

The Replica Set Method: A High-throughput Approach to Quantitatively Measure Caenorhabditis elegans Lifespan

9,395 Views

•

11:58 min

•

June 29, 2018

DOI:

10.3791/57819-v

DOI:

10.3791/57819-v

•

11:58 min

June 29, 2018

•

9370 Views

Adam B. Cornwell¹, Jesse R. Llop¹, Peter Salzman^2,3, Juilee Thakar⁴, Andrew V. Samuelson¹

¹Department of Biomedical Genetics,University of Rochester Medical Center ²Department of Biostatistics and Computational Biology,University of Rochester Medical Center ³Non-Clinical Statistics,Bristol-Myers Squibb ⁴Department of Microbiology and Immunology,University of Rochester Medical Center

Podsumowanie

Here we describe the Replica Set method, an approach to quantitatively measure C. elegans lifespan/survival and healthspan in a high-throughput and robust manner, thus allowing screening of many conditions without sacrificing data quality. This protocol details the strategy and provides a software tool for analysis of Replica Set data.