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February 15, 2019
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Here we present a direct intrathecal injection technique using 1% lidocaine hydrochloride in a viral solution to ensure efficient adeno-associated virus delivery to small animals and establish a scoring system to predict transduction efficiency in the central nervous system according to the degree of transient weakness induced by lidocaine.
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Li, D., Li, Y., Tian, Y., Xu, Z., Guo, Y. Direct Intrathecal Injection of Recombinant Adeno-associated Viruses in Adult Mice. J. Vis. Exp. (144), e58565, doi:10.3791/58565 (2019).
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