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March 14, 2019
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We present a protocol to evaluate the expression levels of circulating microRNA (miRNAs) in plasma samples from cancer patients. In particular, we used a commercially available kit for circulating miRNA extraction and reverse transcription. Finally, we analyzed a panel of 24 selected miRNAs using real-time pre-spotted probe custom plates.
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Canale, M., Marisi, G., Passardi, A., Scarpi, E., Ulivi, P. Detection of a Circulating MicroRNA Custom Panel in Patients with Metastatic Colorectal Cancer. J. Vis. Exp. (145), e58615, doi:10.3791/58615 (2019).
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