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August 21, 2019
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This is a protocol to isolate tissue extracellular vesicles (EVs) from the liver. The protocol describes a two-step process involving collagenase perfusion followed by differential ultracentrifugation to isolate liver tissue EVs.
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Ishiguro, K., Yan, I. K., Patel, T. Isolation of Tissue Extracellular Vesicles from the Liver. J. Vis. Exp. (150), e58649, doi:10.3791/58649 (2019).
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