Using In Vitro Fluorescence Resonance Energy Transfer to Study the Dynamics Of Protein Complexes at a Millisecond Time Scale

Melaku Garsamo; Yun Zhou; Xing Liu

doi:10.3791/59038

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Using In Vitro Fluorescence Resonance Energy Transfer to Study the Dynamics Of Protein Complexes at a Millisecond Time Scale

JoVE Journal
Biochemia

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JoVE Journal Biochemia

Using In Vitro Fluorescence Resonance Energy Transfer to Study the Dynamics Of Protein Complexes at a Millisecond Time Scale

8,008 Views

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10:50 min

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March 14, 2019

DOI:

10.3791/59038-v

DOI:

10.3791/59038-v

•

10:50 min

March 14, 2019

•

4 Views

Melaku Garsamo^1,3, Yun Zhou^2,3, Xing Liu^1,3

¹Department of Biochemistry,Purdue University, ²Department of Botany and Plant Pathology,Purdue University, ³Center for Plant Biology,Purdue University

Transkrypcja

Podsumowanie

Protein-protein interactions are critical for biological systems, and studies of the binding kinetics provide insights into the dynamics and function of protein complexes. We describe a method that quantifies the kinetic parameters of a protein complex using fluorescence resonance energy transfer and the stopped-flow technique.