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Proteome-wide Quantification of Labeling Homogeneity at the Single Molecule Level

Proteome-wide Quantification of Labeling Homogeneity at the Single Molecule Level

JoVE Journal
Biochemia

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JoVE Journal Biochemia

Proteome-wide Quantification of Labeling Homogeneity at the Single Molecule Level

Proteome-wide Quantification of Labeling Homogeneity at the Single Molecule Level

6,083 Views

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08:29 min

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April 19, 2019

DOI:

10.3791/59199-v

DOI:

10.3791/59199-v

•

08:29 min

April 19, 2019

•

2 Views

Simon Leclerc^1,2, Youri Arntz², Yuichi Taniguchi^1,3

¹Laboratory for Cell Systems Control, RIKEN Center for Biosystems Dynamics Research,RIKEN, ²Laboratoire de Biomatériaux et Bioimagerie,INSERM, ³PRESTO, Japan Science and Technology Agency

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Podsumowanie

Here, we present a protocol to assess the labeling homogeneity for each protein species in a complex protein sample at the single molecule level.

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Proteome-wide Quantification Labeling Homogeneity Single Molecule Level Protein Analysis Fluorescent Labeling Diagnostic Methods Molecular Concentration Assays Experimental Reproducibility Lysing Buffer Cy3 NHS Ester Dye Homogenization Biochemistry Protocols Pathogenic Molecules Electrophoresis Chromatography

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