Here, two medium-throughput assays for assessment of effects on Ca2+-signaling and acrosome reaction in human sperm are described. These assays can be used to quickly and easily screen large amounts of compounds for effects on Ca2+-signaling and acrosome reaction in human sperm.
Rehfeld, A., Egeberg Palme, D. L., Almstrup, K., Juul, A., Skakkebaek, N. E. Medium-throughput Screening Assays for Assessment of Effects on Ca2+-Signaling and Acrosome Reaction in Human Sperm. J. Vis. Exp. (145), e59212, doi:10.3791/59212 (2019).