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May 03, 2019
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We successfully converted the standard telomere repeat amplification protocol (TRAP) assay to be employed in droplet digital polymerase chain reactions. This new assay, called ddTRAP, is more sensitive and quantitative, allowing for better detection and statistical analysis of telomerase activity within various human cells.
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Sayed, M. E., Slusher, A. L., Ludlow, A. T. Droplet Digital TRAP (ddTRAP): Adaptation of the Telomere Repeat Amplification Protocol to Droplet Digital Polymerase Chain Reaction. J. Vis. Exp. (147), e59550, doi:10.3791/59550 (2019).
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