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July 01, 2019
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This report describes comprehensive methods for preparing frozen mouse retina sections for immunohistochemistry (IHC). Methods described include dissection of the ocular posterior cup, paraformaldehyde fixation, embedding in Optimal Cutting Temperature (OCT) media and tissue orientation, sectioning and immunostaining.
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Léger, H., Santana, E., Beltran, W. A., Luca, F. C. Preparation of Mouse Retinal Cryo-sections for Immunohistochemistry. J. Vis. Exp. (149), e59683, doi:10.3791/59683 (2019).
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