Using Human Induced Pluripotent Stem Cells for the Generation of Tumor Antigen-specific T Cells

Meghan  L. Good; Raul Vizcardo; Takuya Maeda; Naritaka Tamaoki; Parisa Malekzadeh; Hiroshi Kawamoto; Nicholas  P. Restifo

doi:10.3791/59997

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Badania Nad Rakiem

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Using Human Induced Pluripotent Stem Cells for the Generation of Tumor Antigen-specific T Cells

JoVE Journal
Badania Nad Rakiem

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JoVE Journal Badania Nad Rakiem

Using Human Induced Pluripotent Stem Cells for the Generation of Tumor Antigen-specific T Cells

10,280 Views

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10:57 min

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October 24, 2019

DOI:

10.3791/59997-v

DOI:

10.3791/59997-v

•

10:57 min

October 24, 2019

•

4 Views

Meghan L. Good*^1,2, Raul Vizcardo*^1,2, Takuya Maeda^1,2, Naritaka Tamaoki^1,2, Parisa Malekzadeh¹, Hiroshi Kawamoto³, Nicholas P. Restifo^1,2

¹Surgery Branch,National Cancer Institute, NIH, ²Center for Cell-Based Therapy,National Cancer Institute, NIH, ³Laboratory of Immunology, Institute for Frontier Life and Medical Sciences,Kyoto University

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This article describes a method to generate functional tumor antigen-specific induced pluripotent stem cell-derived CD8αβ⁺ single positive T cells using OP9/DLL1 co-culture system.

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Human Induced Pluripotent Stem Cells Tumor Antigens T Cell Generation CD8 T Cells OP9-Delta-1 Co-culture Regenerative Medicine Cell-based Therapies NK Cells FBS Lot Evaluation IPSC Colonies ROCK Inhibitor Mechanical Flushing Mouse Embryonic Fibroblasts Cell Media Change Confluency Assessment Trypsin-EDTA Incubation

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Using Human Induced Pluripotent Stem Cells for the Generation of Tumor Antigen-specific T Cells

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