Imaging and Analysis of Tissue Orientation and Growth Dynamics in the Developing <em>Drosophila</em> Epithelia During Pupal Stages

Federica Mangione; Enrique Martin-Blanco

doi:10.3791/60282

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Imaging and Analysis of Tissue Orientation and Growth Dynamics in the Developing Drosophila Epithelia During Pupal Stages

Imaging and Analysis of Tissue Orientation and Growth Dynamics in the Developing <em>Drosophila</em> Epithelia During Pupal Stages

JoVE Journal
Biologia Rozwoju

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JoVE Journal Biologia Rozwoju

Imaging and Analysis of Tissue Orientation and Growth Dynamics in the Developing Drosophila Epithelia During Pupal Stages

Imaging and Analysis of Tissue Orientation and Growth Dynamics in the Developing Drosophila Epithelia During Pupal Stages

9,205 Views

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08:25 min

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June 02, 2020

DOI:

10.3791/60282-v

DOI:

10.3791/60282-v

•

08:25 min

June 02, 2020

•

9 Views

Federica Mangione^1,2, Enrique Martin-Blanco¹

¹Instituto de Biología Molecular de Barcelona, Consejo Superior de Investigaciones Científicas,Parc Científic de Barcelona, ²The Francis Crick Institute

Transkrypcja

Podsumowanie

This protocol is designed for the imaging and analysis of the dynamics of cell orientation and tissue growth in the Drosophila abdominal epithelia as the fruit fly undergoes metamorphosis. The methodology described here can be applied to the study of different developmental stages, tissues, and subcellular structures in Drosophila or other model organisms.