9,195 Views
•
08:26 min
•
December 10, 2019
DOI:
Podsumowanie
Automatically generated
Presented here is a protocol for cell culture on silicon nitride membranes and plunge-freezing prior to X-ray fluorescence imaging with a synchrotron cryogenic X-ray nanoprobe. When only room temperature nano-analysis is provided, the frozen samples can be further freeze-dried. These are critical steps to obtain information on the intracellular elemental composition.
Powiązane Filmy
07:37
Revealing Dynamic Processes of Materials in Liquids Using Liquid Cell Transmission Electron Microscopy
Powiązane Filmy
12691 Views
07:54
Preparing Adherent Cells for X-ray Fluorescence Imaging by Chemical Fixation
Powiązane Filmy
9387 Views
11:42
Measurement of Total Calcium in Neurons by Electron Probe X-ray Microanalysis
Powiązane Filmy
12007 Views
09:06
Preparation and Cryo-FIB micromachining of Saccharomyces cerevisiae for Cryo-Electron Tomography
Powiązane Filmy
4307 Views
11:03
Nanoscale Characterization of Liquid-Solid Interfaces by Coupling Cryo-Focused Ion Beam Milling with Scanning Electron Microscopy and Spectroscopy
Powiązane Filmy
3462 Views
10:54
Cryo-electron Microscopy Specimen Preparation By Means Of a Focused Ion Beam
Powiązane Filmy
26592 Views
10:12
Graphene Enclosure of Chemically Fixed Mammalian Cells for Liquid-Phase Electron Microscopy
Powiązane Filmy
7126 Views
08:47
A 3D Cartographic Description of the Cell by Cryo Soft X-ray Tomography
Powiązane Filmy
3879 Views
08:20
Sample Preparation by 3D-Correlative Focused Ion Beam Milling for High-Resolution Cryo-Electron Tomography
Powiązane Filmy
3283 Views
09:22
Direct Synthesis of EM-Visible Gold Nanoparticles in Cells for Protein Localization Analysis with Well-Preserved Ultrastructure
Powiązane Filmy
1411 Views
Read Article
Cytuj ten artykuł
Bissardon, C., Reymond, S., Salomé, M., André, L., Bayat, S., Cloetens, P., Bohic, S. Cell Culture on Silicon Nitride Membranes and Cryopreparation for Synchrotron X-ray Fluorescence Nano-analysis. J. Vis. Exp. (154), e60461, doi:10.3791/60461 (2019).
Copy