Use of Microscale Thermophoresis to Measure Protein-Lipid Interactions

Robert P. Sparks; William Lawless; Andres  S. Arango; Emad Tajkhorshid; Rutilio  A. Fratti

doi:10.3791/60607

Dziennik

/

Biochemia

/

Use of Microscale Thermophoresis to Measure Protein-Lipid Interactions

JoVE Journal
Biochemia

Aby wyświetlić tę treść, wymagana jest subskrypcja JoVE. Zaloguj się lub rozpocznij bezpłatny okres próbny.

JoVE Journal Biochemia

Use of Microscale Thermophoresis to Measure Protein-Lipid Interactions

6,634 Views

•

04:45 min

•

February 10, 2022

DOI:

10.3791/60607-v

DOI:

10.3791/60607-v

•

04:45 min

February 10, 2022

•

15 Views

Robert P. Sparks*¹, William Lawless*², Andres S. Arango*^1,3,4, Emad Tajkhorshid^1,3,4, Rutilio A. Fratti^1,3

¹Department of Biochemistry,University of Illinois at Urbana-Champaign, ²Department of Chemistry,University of South Florida, ³Center for Biophysics and Quantitative Biology,University of Illinois at Urbana-Champaign, ⁴Beckman Institute for Advanced Science and Technology,University of Illinois at Urbana-Champaign

Transkrypcja

Podsumowanie

Microscale thermophoresis obtains binding constants quickly at low material cost. Either labeled or label free microscale thermophoresis is commercially available; however, label free thermophoresis is not capable of the diversity of interaction measurements that can be performed using fluorescent labels. We provide a protocol for labeled thermophoresis measurements.