Discovering CsgD Regulatory Targets in <em>Salmonella</em> Biofilm Using Chromatin Immunoprecipitation and High-Throughput Sequencing (ChIP-seq)

Melissa  B. Palmer; Yejun Wang; Aaron P. White

doi:10.3791/60736

/

Discovering CsgD Regulatory Targets in Salmonella Biofilm Using Chromatin Immunoprecipitation and High-Throughput Sequencing (ChIP-seq)

Discovering CsgD Regulatory Targets in <em>Salmonella</em> Biofilm Using Chromatin Immunoprecipitation and High-Throughput Sequencing (ChIP-seq)

JoVE Journal
Genetyka
Author Produced

This content is Free Access.

JoVE Journal Genetyka

Discovering CsgD Regulatory Targets in Salmonella Biofilm Using Chromatin Immunoprecipitation and High-Throughput Sequencing (ChIP-seq)

Discovering CsgD Regulatory Targets in Salmonella Biofilm Using Chromatin Immunoprecipitation and High-Throughput Sequencing (ChIP-seq)

7,418 Views

•

13:48 min

•

January 18, 2020

DOI:

10.3791/60736-v

DOI:

10.3791/60736-v

•

13:48 min

January 18, 2020

•

4 Views

Melissa B. Palmer^1,2, Yejun Wang³, Aaron P. White^1,2

¹Vaccine and Infectious Disease Organization-International Vaccine Centre, ²Department of Biochemistry, Microbiology and Immunology,University of Saskatchewan, ³Department of Cell Biology and Genetics,Shenzhen University Health Science Center

Transkrypcja

Podsumowanie

Chromatin immunoprecipitation coupled with next-generation sequencing (ChIP-seq) is a method used to establish interactions between transcription factors and the genomic sequences they control. This protocol outlines techniques for performing ChIP-seq with bacterial biofilms, using Salmonella enterica serovar Typhimurium bacterial biofilm as an example.