Taking the Next Step: a Neural Coaptation Orthotopic Hind Limb Transplant Model to Maximize Functional Recovery in Rat

Feibo Zheng; Andy Tully; Kyle M Koss; Xiaomin Zhang; Longhui Qiu; Jiao-Jing Wang; Bilal A Naved; David  Z Ivancic; James M Mathew; Jason  A Wertheim; Zheng  Jenny Zhang

doi:10.3791/60777

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Taking the Next Step: a Neural Coaptation Orthotopic Hind Limb Transplant Model to Maximize Functional Recovery in Rat

JoVE Journal
Medycyna
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JoVE Journal Medycyna

Taking the Next Step: a Neural Coaptation Orthotopic Hind Limb Transplant Model to Maximize Functional Recovery in Rat

7,287 Views

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14:18 min

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August 30, 2020

DOI:

10.3791/60777-v

DOI:

10.3791/60777-v

•

14:18 min

August 30, 2020

•

8 Views

Feibo Zheng^1,3, Andy Tully^1,2, Kyle M Koss¹, Xiaomin Zhang¹, Longhui Qiu¹, Jiao-Jing Wang¹, Bilal A Naved^1,4, David Z Ivancic¹, James M Mathew¹, Jason A Wertheim¹, Zheng Jenny Zhang¹

¹Comprehensive Transplant Center and Department of Surgery, Feinberg School of Medicine,Northwestern University, ²Department of Surgery,University of Illinois at Chicago, ³Department of Surgery,Tianjin Occupational Diseases Precaution and Therapeutic Hospital, ⁴Department of Biomedical Engineering, McCormick School of Engineering,Northwestern University

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Podsumowanie

This protocol presents a robust, reproducible model of vascularized composite allotransplant (VCA) geared toward simultaneous study of immunology and functional recovery. The time invested in meticulous technique in a right mid-thigh hind limb orthotopic transplant with hand sewn vascular anastomoses and neural coaptation yields the ability to study functional recovery.