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March 12, 2020
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The protocol presents a complete validated TNBS-induced murine model of Crohn's disease and methods for visualization of estrogen receptors by immunohistochemistry using immunofluorescence of formalin-fixed colon sections embedded in paraffin.
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Jacenik, D., Zielińska, M., Michlewska, S., Fichna, J., Krajewska, W. M. Visualization of Estrogen Receptors in Colons of Mice with TNBS-Induced Crohn's Disease using Immunofluorescence. J. Vis. Exp. (157), e60813, doi:10.3791/60813 (2020).
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