Isolation and Expansion of Neurospheres from Postnatal (P1&#8722;3) Mouse Neurogenic Niches

Rita Soares; Filipa  F. Ribeiro; Diogo  M. Louren&#231;o; Rui  S. Rodrigues; Jo&#227;o  B. Moreira; Ana  M. Sebasti&#227;o; Vanessa  A. Morais; Sara Xapelli

doi:10.3791/60822

Dziennik

/

Neurologia

/

Isolation and Expansion of Neurospheres from Postnatal (P1−3) Mouse Neurogenic Niches

JoVE Journal
Neurologia

This content is Free Access.

JoVE Journal Neurologia

Isolation and Expansion of Neurospheres from Postnatal (P1−3) Mouse Neurogenic Niches

14,164 Views

•

10:05 min

•

May 23, 2020

DOI:

10.3791/60822-v

DOI:

10.3791/60822-v

•

10:05 min

May 23, 2020

•

23 Views

Rita Soares^1,2,3, Filipa F. Ribeiro^1,2, Diogo M. Lourenço^1,2, Rui S. Rodrigues^1,2, João B. Moreira^1,2, Ana M. Sebastião^1,2, Vanessa A. Morais^1,3, Sara Xapelli^1,2

¹Instituto de Medicina Molecular João Lobo Antunes, Faculdade de Medicina,Universidade de Lisboa, ²Instituto de Farmacologia e Neurociências, Faculdade de Medicina,Universidade de Lisboa, ³Instituto de Biologia Molecular, Faculdade de Medicina,Universidade de Lisboa

Transkrypcja

Podsumowanie

In this article, we describe, in detail, a protocol for the generation of neurosphere cultures from postnatal mouse neural stem cells derived from the main mouse neurogenic niches. Neurospheres are used to identify neural stem cells from brain tissue allowing the estimation of precursor cell numbers. Moreover, these 3D structures can be plated in differentiative conditions, giving rise to neurons, oligodendrocytes and astrocytes, allowing the study of cell fate.