Presented here is a protocol to study the coronary microcirculation in living murine heart tissue by ex vivo monitoring of the arterial perfusion pressure and flow that maintains the pressure, as well as vascular tree components including the capillary beds and pericytes, as the septal artery is cannulated and pressurized.
Zhao, G., Joca, H. C., Lederer, W. J. Dynamic Measurement and Imaging of Capillaries, Arterioles, and Pericytes in Mouse Heart. J. Vis. Exp. (161), e61566, doi:10.3791/61566 (2020).