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Analysis of SEC-SAXS data via EFA deconvolution and Scatter
Analysis of SEC-SAXS data via EFA deconvolution and Scatter
JoVE Journal
Biochemia
This content is Free Access.
JoVE Journal Biochemia
Analysis of SEC-SAXS data via EFA deconvolution and Scatter

Analysis of SEC-SAXS data via EFA deconvolution and Scatter

English

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10:59 min

January 28, 2021

DOI:

10:59 min
January 28, 2021

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Podsumowanie

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SEC-BioSAXS measurements of biological macromolecules are a standard approach for determining solution structure of macromolecules and their complexes. Here, we analyze SEC-BioSAXS data from two types of commonly encountered SEC traces—chromatograms with fully resolved and partially resolved peaks. We demonstrate the analysis and deconvolution using scatter and BioXTAS RAW.

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