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February 08, 2021
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We describe a single-molecule approach to antigen-antibody affinity measurements using mass photometry (MP). The MP-based protocol is fast, accurate, uses a very small amount of material, and does not require protein modification.
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Wu, D., Piszczek, G. Rapid Determination of Antibody-Antigen Affinity by Mass Photometry. J. Vis. Exp. (168), e61784, doi:10.3791/61784 (2021).
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