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October 23, 2021
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Immunohistochemistry staining and 16S ribosomal RNA gene (16S rRNA gene) sequencing were performed in order to discover and distinguish bacteria in cancerous and noncancerous ovarian tissues in situ. The compositional and functional differences of the bacteria were predicted by using BugBase and Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt).
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Zhao, L., Zhao, W., Wang, Q., Liang, D., Liu, Y., Fu, G., Han, L., Wang, Y., Sun, C., Wang, Q., Song, Q., Li, Q., Lu, Q. Characterization and Functional Prediction of Bacteria in Ovarian Tissues. J. Vis. Exp. (176), e61878, doi:10.3791/61878 (2021).
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