Single Molecule Fluorescence Energy Transfer Study of Ribosome Protein Synthesis

Yuhong Wang

doi:10.3791/62664

Dziennik

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Biochemia

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Single Molecule Fluorescence Energy Transfer Study of Ribosome Protein Synthesis

JoVE Journal
Biochemia

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JoVE Journal Biochemia

Single Molecule Fluorescence Energy Transfer Study of Ribosome Protein Synthesis

2,591 Views

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08:07 min

•

July 06, 2021

DOI:

10.3791/62664-v

DOI:

10.3791/62664-v

•

08:07 min

July 06, 2021

•

1 Views

Yuhong Wang¹

¹Department of Biology and Biochemistry,University of Houston

Transkrypcja

Podsumowanie

Single molecule fluorescence energy transfer is a method that tracks the tRNA dynamics during ribosomal protein synthesis. By tracking individual ribosomes, inhomogeneous populations are identified, which shed light on mechanisms. This method can be used to track biological conformational changes in general to reveal dynamic-function relationships in many other complexed biosystems. Single molecule methods can observe non-rate limiting steps and low-populated key intermediates, which are not accessible by conventional ensemble methods due to the average effect.