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December 09, 2022
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The present protocol describes the extraction of non-protein amino acids from biological matrices via trichloroacetic acid (TCA) protein precipitation and acid hydrolysis before analysis using liquid chromatography-tandem mass spectrometry.
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Pravadali-Cekic, S., Violi, J. P., Mitrovic, S. M., Rodgers, K. J., Bishop, D. Extraction of Non-Protein Amino Acids from Cyanobacteria for Liquid Chromatography-Tandem Mass Spectrometry Analysis. J. Vis. Exp. (190), e63779, doi:10.3791/63779 (2022).
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