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June 23, 2022
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We have previously developed protocols for Caenorhabditis elegans to form short- and long-term associative memories by massed and spaced training, respectively. Here, detailed protocols are described for the conditioning of C. elegans by pairing 1-propanol and hydrochloric acid as conditioned and unconditioned stimuli, respectively, to form aversive associative memory.
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Shibutani, M., Vibulyaseck, S., Maruyama, I. N. Aversive Associative Learning and Memory Formation by Pairing Two Chemicals in Caenorhabditis elegans. J. Vis. Exp. (184), e64137, doi:10.3791/64137 (2022).
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