This protocol offers a method to study cellular dynamics using a simple in vitro culture technique. It provides an opportunity for zebrafish researchers and educators to study cellular processes, such as those related to bone homeostasis and basic cell biology, by visualizing fluorescent nuclei and apoptotic cells within the scales.
Carvajal-Agudelo, J. D., Franz-Odendaal, T. A. In Vitro Culturing Technique for Studying Cellular Dynamics in Zebrafish Scales. J. Vis. Exp. (215), e66619, doi:10.3791/66619 (2025).