25,288 Views
•
11:57 min
•
May 23, 2008
DOI:
Podsumowanie
Automatically generated
Mycobacterial pathogenic strategies remain poorly understood. The slow growth rate of most species, the impenetrable nature of the cell-wall, and the hazards of working with pathogens make mycobacteria difficult to study and are largely responsible for our poor understanding of these organisms. In this video we will demonstrate the technique of electroporation, which involves subjecting cells to a brief high electrical impulse to allow the entry of DNA. It is the most widely used method for introducing DNA into mycobacterial cells.
Powiązane Filmy
19:04
In Utero Intraventricular Injection and Electroporation of E16 Rat Embryos
Powiązane Filmy
15834 Views
26:17
In Utero Intraventricular Injection and Electroporation of E15 Mouse Embryos
Powiązane Filmy
27675 Views
15:56
DNA Transfection of Mammalian Skeletal Muscles using In Vivo Electroporation
Powiązane Filmy
17725 Views
12:29
Primary Culture and Plasmid Electroporation of the Murine Organ of Corti.
Powiązane Filmy
25770 Views
07:23
Electroporation of Craniofacial Mesenchyme
Powiązane Filmy
11801 Views
06:55
In vivo Electroporation of Morpholinos into the Adult Zebrafish Retina
Powiązane Filmy
19102 Views
07:22
In vivo Electroporation of Morpholinos into the Regenerating Adult Zebrafish Tail Fin
Powiązane Filmy
16848 Views
06:15
In Ovo Electroporation in Embryonic Chick Retina
Powiązane Filmy
14315 Views
06:10
Gene Transfer into Older Chicken Embryos by ex ovo Electroporation
Powiązane Filmy
14503 Views
08:39
In Vivo Microinjection and Electroporation of Mouse Testis
Powiązane Filmy
27749 Views
Read Article
Cytuj ten artykuł
Goude, R., Parish, T. Electroporation of Mycobacteria. J. Vis. Exp. (15), e761, doi:10.3791/761 (2008).
Copy