Name: blast practice for fossil dna clues
Uploaded: 2019-01-31T09:26:12.000Z
Duration: 1 min 2 s
Channel: JoVE

K12 Standards

Virginia  Standards of Learning

HIgh School

Biology

BIO.7

BIO.7.d

Evolutionary Relationships

<ul><li><a href="#" data-time="0.0">Usando o BLAST para testar hipóteses evolutivas
</a></li></ul>

<ul class="lab-items"><li data-lab-item="1">
								<div class="ingrediente" data-value="Imagem de cladograma em branco"><a target="_blank" href="/files/ftp_upload/10562/Blank_Cladogram.png">Imagem de cladograma em branco</a></div>
								<div class="ingrediente-quantidade">
									<span class="amount" data-value="10">10</span>
									<span class="unit" data-value=""></span>
								</div>
							</li><li data-lab-item="1">
								<div class="ingrediente" data-value="Computador com conexão à internet">Computador com conexão à internet</div>
								<div class="ingrediente-quantidade">
									<span class="amount" data-value="-1">-1</span>
									<span class="unit" data-value="Dependente do tamanho do laboratório">Dependente do tamanho do laboratório</span>
								</div>
							</li></ul>

relações evolutivas

<ol class="note-items"><li data-note-item="1" data-parent-collapse="">Usando o BLAST para testar hipóteses evolutivas
<button class="expand">Expand</button><ul class="lab-items"><li data-sub-note-item="1-1"><div class="lab-step">Abra o site do BLAST em cada computador que os alunos usarão. <a target="__blank" href="https://blast.ncbi.nlm.nih.gov/Blast.cgi" style="display:block;">https://blast.ncbi.nlm.nih.gov/Blast.cgi</a>
</div></li><li data-sub-note-item="1-2"><div class="lab-step">Navegue até o link fornecido para encontrar as sequências genéticas de frango, jacaré e tentilhão-zebra. <a href="https://github.com/EvolutionGenes/Evolutionary-Relationships-Genes" target="__blank" style="display:block;">https://github.com/EvolutionGenes/Evolutionary-Relationships-Genes</a> NOTA: Essas sequências de genes serão usadas como amostras de DNA relativas vivas para o fóssil misterioso.
</div></li><li data-sub-note-item="1-3"><div class="lab-step">Clique na primeira sequência para se familiarizar com ela. 
</div></li><li data-sub-note-item="1-4"><div class="lab-step">Retorne à página principal e clique no botão verde "Clonar ou baixar". 
</div></li><li data-sub-note-item="1-5"><div class="lab-step">Em seguida, selecione 'Baixar ZIP'. 
</div></li><li data-sub-note-item="1-6"><div class="lab-step">Salve os arquivos na área de trabalho do computador para que os alunos acessem facilmente. Repita as etapas 2 a 6 em cada computador. 
</div></li><li data-sub-note-item="1-7"><div class="lab-step">Imprima um número suficiente de cladogramas em branco para que haja um por aluno.  NOTA: Recomenda-se também imprimir cladogramas sobressalentes.
</div></li></ul></li></ol>

<div class="section-question"><h4 class="question-sect">O que são sinapomorfias?</h4><p>As sinapomorfias são características físicas mensuráveis ou quantificáveis que podem ser usadas para distinguir e dividir grupos de organismos vivos.
</p></div><div class="section-question"><h4 class="question-sect">O que são dendrogramas?</h4><p>Um dendrograma é um diagrama de árvore que representa as relações evolutivas ou cladísticas entre grupos de organismos.
</p></div><div class="section-question"><h4 class="question-sect">Quais são os dois tipos princ...

Relações evolutivas

Usando o BLAST para testar hipóteses evolutivas
ExpandirAbra o site do BLAST em cada computador que os alunos usarão. ...

Education

JoVE Lab Manual

Biologia

Evolution

Evolução

Lab Manual Sub Articles

Subartigos do Manual de Laboratório

blast practice for fossil dna clues

<p class="jove_content">Students use BLAST to test evolutionary hypotheses by comparing DNA sequences. BLAST, or Basic Local Alignment Search Tool, helps match a gene sequence to similar sequences in a database. In this activity, the comparison is used to investigate a mystery fossil and its possible living relatives.</p>

<p class="jove_content">The setup begins with the BLAST website opened on each student computer. Students then use the provided link to find the chicken, alligator, and zebra finch gene sequences. These sequences serve as the living relative DNA samples for the mystery fossil.</p>

<p class="jove_content">To prepare the files, the first sequence is opened so students can become familiar with it. Then the main page is used to click the green “Clone or download” button and choose “Download ZIP.” The files are saved on the desktop so students can access them easily. The same steps are repeated on each computer.</p>

<p class="jove_content">Blank cladograms are also printed for the class. Each student should have one cladogram to complete, and extra copies are recommended. The activity gives students a way to compare DNA evidence and build a simple model of evolutionary relationships.</p>

BLAST Practice for Fossil DNA Clues

evolutionary relationships

Testing Fossil Placement with Cladograms

Cladograms and BLAST help students test where a fossil may fit on an evolutionary tree. A cladogram is a tree-shaped chart that shows hypothetical genealogical relationships between species. The tips, or leaves, show specific species, and the branch lengths show the degree of change between them. A node is the point where branches meet and marks the common ancestor of the species that split from that line. Species that share a node are called a sister group.<br /><br />To start, students examine a blank...

Students use BLAST to test evolutionary hypotheses by comparing DNA sequences. BLAST, or Basic Local Alignment Search Tool, helps match a gene sequence to similar sequences in a database. In this activity, the comparison is used to investigate a mystery fossil and its possible living relatives.<br /><br />The setup begins with the BLAST website opened on each student computer. Students then use the provided link to find the chicken, alligator, and zebra finch gene sequences. These sequences serve as the...

lab manual sub articles

Set up a BLAST-based class activity with chicken, alligator, and zebra finch DNA samples to compare a mystery fossil and its living relatives.

BLAST Practice for Fossil DNA Clues | JoVE K12

Next Generation Science Standards

High School

Life Science

heredity-inheritance-and-variation-of-traits

Heredity: Inheritance and Variation of Traits

HS-LS3-3

HS-LS4-1

Florida Department of Education

SC.912.L.15

SC.912.L.15.4

SC.912.L.15.10

new-york-state-science-learning-standards

New York State Science Learning Standards

Inheritance and Variation of Traits

HS-LS3-3.

Natural Selection and Evolution

HS-LS4-1.

Georgia Standards of Excellence

9-12

SB4.b

<p>Os humanos classificaram e organizaram organismos biológicos por milhares de anos. Originalmente, principalmente ordenando objetos necessários para a sobrevivência. À medida que a história humana progredia, o mesmo acontecia com a habilidade e os detalhes dessas classificações. No século IV a.C., Aristóteles foi pioneiro em classificações formais, delineando plantas e animais em diferentes grupos e, em seguida, dividindo-os ainda mais com base em suas características físicas e traços, como os habitats que ocupam. Mais tarde, em meados de 1700, Linnaeus construiu o sistema de Aristóteles. Ele chamou seu nível mais alto de agrupamento dos reinos e, a partir daí, dividiu os grupos usando sinapomorfias, uma característica física definidora que divide um ramo. Por exemplo, se um animal possui uma espinha dorsal ou uma estrutura semelhante, ele deve ser colocado no filo chordata. Se isso não acontecer, então existem muitos outros filos, nos quais os animais sem espinha dorsal podem ser divididos, incluindo os artrópodes, um grande grupo que inclui insetos. Linneaus continuou dividindo grupos de organismos com base em suas sinapomorfias em níveis subsequentes através da classe, ordem, família e gênero, até chegar à designação final, geralmente, a espécie. Referimo-nos ao tipo de classificação de Linnaeus como cladística, a classificação de organismos com base em diferenças nas características físicas.</p>
<p>Hoje, os cientistas geralmente constroem árvores chamadas dendrogramas para fornecer representações visuais dessas divisões e grupos. Essa forma particular de dendrograma, o cladograma, visualiza as relações cladísticas entre as espécies para que as pontas da árvore representem as espécies e os galhos mostrem como eles estão relacionados entre si. Por exemplo, aqui o chimpanzé e o urso estão mais intimamente relacionados entre si e compartilham características mais comuns do que qualquer um deles com o peixe-lua. Os locais onde os ramos se encontram são chamados de nós e denotam ancestrais comuns para as espécies que se seguem. Um segundo tipo principal de dendrograma é o filograma. Estes são diferentes dos cladogramas porque o comprimento dos ramos entre as espécies varia, representando o grau de mudança entre eles. Portanto, quanto mais longo o galho, mais tempo se passou desde que a espécie divergiu de seu último ancestral comum.</p>
<p>Os dendrogramas foram construídos simplesmente analisando a morfologia dos organismos. Com o advento da tecnologia moderna, comparar o DNA também se tornou uma maneira comum de construir árvores. O DNA é composto de nucleotídeos associados a uma das quatro bases diferentes. Adenina, guanina, citosina ou timina. A ordem dessas bases é o código do DNA. Este código é passado de pais para filhos. Consequentemente, se você olhar para uma única espécie como os humanos, há um grau muito alto de semelhança em nosso código genético, em torno de 99,9%. Também compartilhamos parte do nosso código de DNA com outras espécies, como chimpanzés e camundongos, mas o grau de semelhança geral entre nosso DNA e o deles é muito diferente. Isso significa que podemos criar árvores, que agrupam espécies com base nas semelhanças ou diferenças entre seus códigos genéticos. Este campo de análise, combinando estatística, modelagem matemática e ciência da computação, é conhecido como bioinformática. Para comparar sequências de DNA, os pesquisadores costumam usar uma ferramenta de bioinformática chamada Basic Local Alignment Search Tool, ou BLAST, que foi criada e é mantida pelo Centro Nacional de Informações sobre Biotecnologia.</p>
<p>Neste laboratório, você primeiro criará um cladograma de animais usando informações morfológicas e, em seguida, colocará uma espécie fóssil neste cladograma com base em sua morfologia. Em seguida, você usará sequências de DNA de vários parentes modernos diferentes do fóssil e o banco de dados BLAST para verificar o posicionamento do fóssil na árvore.</p>

dna evidence in evolutionary trees

<p class="jove_content">DNA evidence helps scientists map evolutionary relationships between living things. Early classification began with Aristotle in the 4th century BC and was later improved during the Renaissance and by Carolus Linnaeus in the mid-1700s. These systems grouped species by physical similarity, such as the presence or absence of a backbone in vertebrates and invertebrates. Traits like a backbone are called synapomorphies, which are shared traits that likely came from a common ancestor.</p>

<p class="jove_content">Physical traits are still useful, but they also have limits. To show how species are related, scientists build dendrograms, or branching diagrams. A cladogram is one type of dendrogram. It shows hypothetical family-like relationships, with the tips representing species and the branches showing how they are connected.</p>

<p class="jove_content">A phylogram is a more detailed tree. Its branch lengths are not the same. The length of a branch shows the amount of change between species, so a longer branch means more time since two species diverged from a common ancestor. In both cladograms and phylograms, a node marks the point where branches meet. Species that share the most recent common ancestor are closest to that node and are called sister groups.</p>

<p class="jove_content">Scientists used to make cladograms by comparing morphology, or body structure. That method is still used, but it now includes DNA sequence comparison. DNA evidence can also help estimate how long ago species shared a common ancestor. This is especially useful for understanding evolutionary history, although it does not always work for extinct organisms because DNA is usually found in soft tissue, and soft tissue is rarely preserved in fossils.</p>

<p class="jove_content">Genes carry DNA from parents to offspring. The nucleotide sequence in genes uses the bases A, G, C, and T. These sequences are often similar in different species because of common ancestry. Researchers can align them to build trees. Species with more similar sequences are placed closer together, while species with less similarity are placed farther apart.</p>

<p class="jove_content">Bioinformatics tools help scientists work with large DNA datasets. These tools combine computer science, math, and statistics. One example is BLAST, or Basic Local Alignment Search Tool. BLAST searches the NCBI database, which brings together several databases of DNA sequence information. It compares a query sequence against sequences in the database and lists the closest matches first.</p>

<p class="jove_content">A BLAST result can suggest which species are closely related to the source of the query gene. However, this comparison may not always show the exact evolutionary relationship. Genes can evolve at different rates, and genomes can contain more than one similar sequence. Even so, DNA comparisons are valuable for more than tree building. Scientists often use genes first found in model organisms such as the fruit fly Drosophila melanogaster or the mouse to study gene function in other species, including humans. Human genes can also be used to find homologs in model organisms. This approach is important in human disease research. For example, researchers can knock out a mouse version of a human gene to create disease models such as the Cftr knockout mouse for cystic fibrosis or the Apoe knockout mouse for atherosclerosis.</p>

DNA Evidence in Evolutionary Trees

Os humanos têm tentado classificar adequadamente os seres vivos desde que Aristóteles fez a primeira tentativa durante o século 4 aC. O sistema de Aristóteles ...

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Phylogenetic Trees

Phylogenetic trees come in many forms. It matters in which sequence the organisms are arranged from the bottom to the top of the tree, but the branches ...

Conditions on Early Earth

Around 4 billion years ago, oceans began to condense on earth while volcanic eruptions released nitrogen, carbon dioxide, methane, ammonia, and hydrogen ...

The Colonization of Land

Changes in the environment of the early Earth drove the evolution of organisms. As prokaryotic organisms in the oceans began to photosynthesize, they ...

What is Evolutionary History?

Scientists record evolutionary history by analyzing fossil, morphological, and genetic data. The fossil record documents the history of life on Earth and ...

The Evidence for Evolution

Genetic variations accumulating within populations over generations give rise to biological evolution. Evolutionary changes can result in the formation of ...

The Fossil Record

The fossil record documents only a small fraction of all organisms that have ever inhabited Earth. Fossilization is a rare process, and most organisms ...

Convergent Evolution

Evolution shapes the features of organisms over time, ensuring that they are suited for the environments in which they live. Sometimes, selection pressure ...

The student will investigate and understand that populations change through time. Key ideas include  evidence is found in fossil records and through DNA analysis;

BIO.7.a

What are Populations and Communities?

Populations are groups of individuals of the same species that inhabit a shared environment. Communities include multiple co-existing, interacting ...

Distribution and Dispersion

Ecology is the study of how organisms interact with their environment and with one another. An important aspect of ecology is understanding where species ...

Life Histories

Constrained by limited energy and resources, organisms must compromise between offspring quantity and parental investment. This trade-off is represented ...

Population Growth

Population size is dynamic, increasing with birth rates and immigration, and decreasing with death rates and emigration. In ideal conditions with ...

Ecological Niches

All organisms have a position within an ecosystem. The complete set of living and nonliving factors&#x2014;including food resources, climate, and ...

Ecological Succession

Ecological succession is influenced by the processes of facilitation, inhibition, and toleration. Facilitation occurs when early successional species ...

Keystone Species

Measures of species biodiversity, such as richness (i.e., the number of species present) and evenness (i.e., their relative abundance), describe an ...

Symbiosis

Symbiotic relationships are long-term, close interactions between individuals of different species that affect the distribution and abundance of those ...

Competition

When organisms require the same limited resources within an environment, they may have to compete for them. Competition is a net-negative interaction. ...

Predator-Prey Interactions

Predators consume prey for energy. Predators that acquire prey and prey that avoid predation both increase their chances of survival and reproduction ...

Ecological Disturbance

An ecological disturbance is a temporary disruption in the environment resulting from abiotic, biotic, or anthropogenic factors, causing a pronounced ...

The student will investigate and understand that populations change through time. Key ideas include  genetic variation, reproductive strategies, and environmental pressures affect the survival of populations;

BIO.7.b

What is Natural Selection?

Natural selection is an evolutionary process in which individuals with survival-promoting traits reproduce at higher rates. These favorable traits become ...

Types of Selection

Natural selection influences the frequencies of particular alleles and phenotypes within populations in several different ways. Primarily, natural ...

Frequency-dependent Selection

When the fitness of a trait is influenced by how common it is (i.e., its frequency) relative to different traits within a population, this is referred to ...

Limits to Natural Selection

Organisms that are well-adapted to their environment are more likely to survive and reproduce. However, natural selection does not lead to perfectly ...

Natural Selection

Fitness
Widespread variation of phenotypes in natural populations provides the raw material for evolution, which is the change in the inherited traits of ...

Simulating Natural Selection
ExpandTo prepare for the lab, set out workstations for each student pair containing 6 white, 6 black, and 13 gray 12 inch ...

Simulating Natural Selection
ExpandTo begin, cut the white, black, and gray pipe cleaners into quarters, which should produce a total of 100 pieces that ...

Artificial Selection

Natural Selection and Adaptive Evolution
In natural settings, adaptive evolution takes place by natural selection, a process in which differences in ...

Observing Artificial Selection in Plants
ExpandTwo weeks before beginning the pollination experiments, collect a sufficient amount of small pots with a ...

Observing Artificial Selection in Plants 
ExpandTo begin the experiment, collect five two-week old grown plants from the generation one population that ...

The student will investigate and understand that populations change through time. Key ideas include  natural selection is a mechanism that leads to adaptations and may lead to the emergence of new species; and

BIO.7.c

Humans have been attempting to properly classify living things since Aristotle made the first attempt during the 4th century BC. Aristotle&#8217;s system ...

Using BLAST to Test Evolutionary Hypotheses
ExpandOpen the BLAST website on each computer that the students will be using. ...

Forming an Evolutionary Hypothesis
ExpandNOTE: A cladogram is an important tool for forming an evolutionary hypothesis. A cladogram is a tree-shaped chart ...

The student will investigate and understand that populations change through time.Key ideas include  biological evolution has scientific evidence and explanations.

BLAST Practice for Fossil DNA Clues

Learning Objectives

O que são sinapomorfias?

O que são dendrogramas?

Lab Prep

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