Name: how genome size shapes new genes
Uploaded: 2020-11-23T11:47:33.000Z
Duration: 3 min 21 s
Channel: JoVE

K12 Standards

north-carolina-k-12-science-standard-course-of-study

NC K-12 Science Standard Course of Study

9-12

Biology

Biological Evolution- Unity and Diversity

LS.Bio.9.2

genome-size-and-the-evolution-of-new-genes

Genome Size and the Evolution of New Genes

Apesar da natureza simples do código genético, há uma variação considerável em termos do tamanho do genoma, do menor genoma conhecido, incluindo a proteobacteria Candidatus Carsonella ruddii com menos de 160, 000 pares base, aos maiores, como a planta de flores japonesa Paris japonica com cerca de 150 bilhões. Apesar destes extremos, bactérias e archaea geralmente têm cerca de 3, 000 genes dentro de seus genomas. Porque os procariotas têm quase nenhuma sequência de não codificação, isto significa que seus genomas podem ser relativamente pequenos comparados com aqueles de eucariotas.Genomas menores também significa menos para replicar em cada ronda de divisão de célula, que faz sentido logístico para uma reprodução rápida. Eucariotas tipicamente tem algo próximo do número de 20, 000 genes, mas seus genomas são pontuados por longos períodos da sequência de não codificação, significando que em genoma, o tamanho não necessariamente traduz para complexidade. O genoma da Paris japonica pode ser mais de 50 vezes o tamanho do genoma humano, mas isso é devido pelo menos em parte pela vasta quantidade de sequências de não codificação e provavelmente alto nível de duplicação, não necessariamente mais genes novos.Então como os organismos desenvolvem novos genes? A resposta é geralmente modificando a sequência que já existe. Um dos principais recursos para que novos genes evoluam é através da duplicação de genes.Imagine uma seção de DNA contendo um gene é acidentalmente duplicado. Agora o organismo tem uma segunda cópia de um gene existente. Estas novas cópias genéticas são livres das restrições colocadas na original para manter a função.E assim eles podem divergir, potencialmente desenvolvendo uma nova função ou uma diferente da função original. Outra forma de criar um novo gene são recombinações do DNA, onde segmentos de um gene existente ou cópia gênica são separados e movem-se para aderir os de um gene diferente, fazendo um gene híbrido que pode assumir uma nova função. Mutação intragênica, as alterações numa sequência genética introduzida por mutações ao longo do tempo, é responsável por muitos novos genes.Esta divergência é mais percetível ao comparar espécies ou linhagens que são divergentes, independentemente. Uma vez que esta divergência vai além de um determinado ponto ou um gene assume uma nova função. Eles podem ser classificados como genes diferentes inteiramente.Finalmente, a transferência genética horizontal traz um novo gene ou sequências genes para o genoma de fontes externas, como outros indivíduos e mesmo outras espécies. Este tipo de nova aquisição genética é o mais comum dentro procariotas e archaea com a transferência de genes de resistência aos antibióticos sendo um exemplo bem conhecido. Enquanto raro em eucariotas, ainda é considerada como fonte essencial de novidade genética.E material genético pode até vir de espécies distalmente relacionadas, como as bactérias e fungos neste exemplo.

tamanho do genoma e evolução de novos genes

Embora todo organismo vivo tenha algum tipo de genoma (seja RNA ou DNA), há uma variação considerável nos tamanhos desses projetos. Um fator importante que afeta o tamanho do genoma é se o organismo é procarionte ou eucarionte. Nos procariontes, o genoma contém pouca ou nenhuma sequência não codificante, de modo que os genes estão fortemente agrupados em grupos ou operons ao longo do cromossomo. Por outro lado, os genes nos eucariontes são pontuados por longos trechos de sequências não codificantes. No geral, isso contribui para o fenômeno de que os genomas procariontes tendem a ser menores (isto é, contêm menos bases) em média do que os dos eucariontes. Não é novidade que, dada esta observação, os menores genomas conhecidos são principalmente procariontes. Candidatus Carsonella rudii, por exemplo, é uma proteobactéria altamente simplificada que possui um genoma de apenas 160 mil pares de bases. Tendo perdido muitos genes necessários para sintetizar proteínas essenciais à vida, evoluiu para ser um simbionte intracelular obrigatório. No extremo oposto do espectro, a planta eucarionte japonesa Paris japonica é um dos maiores genomas conhecidos, com cerca de 150 mil milhões de pares de bases. Embora o número de genes que codifica não seja conhecido, o genoma mostra grandes quantidades de duplicação e sequências não codificantes. Dentro do genoma de um procarionte médio existem cerca de 3.000 genes. O eucarionte médio tem em torno de 20.000. Mas o tamanho do genoma, especialmente em eucariontes, é extremamente variável – em grande parte devido à quantidade de sequência não codificante. A criação de novos genes Para que novos genes evoluam, os organismos têm algumas opções principais. A única coisa que a maioria deles têm em comum é que modificam sequências que já existem. A duplicação desempenha um papel importante na criação de novos genes e existem alguns tipos de duplicação que podem resultar nessas novas sequências. Na duplicação de genes, uma seção de DNA contendo um gene é duplicada. Esta segunda cópia não enfrenta a pressão de seleção que restringe a primeira e, por isso, pode divergir. Com o tempo, isso pode levar à evolução de novos genes, com novas funções. Outro tipo de duplicação – embaralhamento de DNA – pode resultar na duplicação de apenas uma seção de um gene e na junção de outro gene. Isso pode resultar na criação de novos genes, com novos produtos. Às vezes, novos genes simplesmente evoluem a partir de mutações acumuladas ao longo do tempo. Isso é conhecido como mutação intragênica e é mais perceptível quando se compara espécies ou populações divergentes. Por fim, também é possível obter novos genes de fontes externas, em um processo conhecido como transferência horizontal de genes. Isto significa que o material genético pode ser incorporado de outros indivíduos, às vezes da mesma espécie, mas também potencialmente de outra espécie. Esta é uma fonte frequente de novos genes em procariontes e arqueias. É menos comum em eucariontes, mas foi demonstrado que ocorre, e os eucariontes podem até obter informações genéticas de fontes tão distantes como bactérias ou fungos.

Tamanho do Genoma e Evolução de Novos Genes

Embora todo organismo vivo tenha algum tipo de genoma (seja RNA ou DNA), há uma variação considerável nos tamanhos desses projetos. Um dos principais ...

Education

JoVE Core

Molecular Biology

Biologia Molecular

Unit 1

Unidade 1

DNA, Cells, and Evolution

DNA, Células e Evolução

KEY TERMS AND CONCEPTS

TERMOS-CHAVE E CONCEITOS

how genome size shapes new genes

Genome size can vary widely, and it also helps show how new genes form. Every living organism has a genome, whether it uses RNA or DNA. Yet the size of that genome can be very different from one organism to another.

One major difference is whether the organism is prokaryotic or eukaryotic. Prokaryotic genomes usually have little to no non-coding sequence, which means genes are packed closely together in operons along the chromosome. Eukaryotic genomes contain long stretches of non-coding DNA between genes. This helps explain why prokaryotic genomes are often smaller than eukaryotic genomes.

The smallest known genomes are mostly prokaryotic. One example is Candidatus Carsonella rudii, a highly simplified proteobacterium with a genome of only 160 thousand base-pairs. It has lost many genes needed to make life-essential proteins, so it now lives as an obligate intracellular symbiont. On the other end of the range, the Japanese flowering plant Paris japonica has one of the largest known genomes, at about 150 billion base-pairs. Its genome contains extensive duplication and non-coding sequence, and the number of genes it encodes is not known.

Gene counts also differ between these groups. An average prokaryote has roughly 3,000 genes. An average eukaryote has around 20,000 genes. Even so, genome size in eukaryotes can vary a great deal because of how much non-coding DNA they contain.

New genes usually arise by changing sequences that already exist. Gene duplication copies a DNA segment that contains a gene. The extra copy is not under the same selection pressure as the original, so it can change over time and take on a new role. DNA shuffling can duplicate only part of a gene and join it to another gene, which can create a new gene product.

New genes can also appear through intragenic mutation, which means mutations build up within a gene over time. This is often seen when comparing different species or populations that have diverged. Another source is horizontal gene transfer, where genetic material comes from another individual or even another species. This is a common way for prokaryotes and archaea to gain novel genes, although it can also happen in eukaryotes, including gene transfers from bacteria or fungi.

How Genome Size Shapes New Genes

the dna helix

DNA Structure and Base Pairing

DNA has a helical structure that helps store genetic information. The double helix is the shape formed by two strands that twist around each other. This structure is a key feature of DNA in cells. The two strands are held together by base pairing. Bases on one strand match with bases on the other strand in a regular way. This pairing helps keep the DNA molecule stable and organized. The helix shape also helps explain how DNA fits into chromosomes and carries instructions for living things. By...

the central dogma

DNA to Protein: Central Dogma Steps

The central dogma explains how genetic information moves from DNA to RNA to protein. This flow is a key idea in molecular biology. It helps show how cells use genes to make the molecules that carry out work. DNA stores the instructions for making proteins. During transcription, a gene in DNA is copied into RNA. The RNA then carries that message to the next stage. In translation, the RNA message is used to build a protein. Proteins do much of the cell’s work, so this process connects genes to...

prokaryotic cells

Inside Prokaryotic Cell Structure

Prokaryotic cells have a simple internal structure compared with many other cells. They are usually divided into two groups: bacteria and archaea. These cells have no nucleus, so their DNA is not enclosed in a membrane-bound compartment. Instead, the genetic material is found in the nucleoid, a region where the DNA is located in the cell. Some prokaryotic cells also contain plasmids, which are small extra DNA molecules. A capsule may surround the cell and provide an additional outer layer. <br...

eukaryotic compartmentalization

Cell Compartments in Eukaryotes

Eukaryotic cells use compartments to organize many different jobs inside one cell. These compartments help separate processes so they can happen in the right place. The compartment called the cytosol is the fluid part of the cell. It surrounds the other internal structures and helps support many cell processes. Inside the cell, membrane-bound organelles create distinct microenvironments. A microenvironment is a small internal space with conditions that fit a specific task. This separation...

the tree of life - bacteria, archaea, eukaryotes

the-tree-of-life-bacteria-archaea-eukaryotes

How Life Is Classified Into Three Domains

The tree of life shows how organisms are related through evolution. Its root represents the common ancestor of all life on Earth. From that point, branches spread out like a tree. The tips of the branches represent living, or extant, species. Species that no longer exist, called extinct species, appear closer to the center of the tree. Today, these organisms are grouped into three domains: bacteria, archaea, and eukaryotes. Scientists still debate these divisions, so the tree of life keeps...

mutations

How Mutations Change DNA

Mutations are changes in DNA. They can alter the genetic code and affect how genes work. In high school biology, students often study mutations to understand how DNA changes can influence traits. The transcript focuses on two common mutation types: point mutations and frameshift mutations. A point mutation changes one nucleotide, which is one DNA building block. A frameshift mutation happens when nucleotides are added or removed and the reading frame shifts. These changes matter because they...

gene evolution - fast or slow?

Why Some Gene Regions Evolve Faster

Gene regions do not all change at the same rate. In eukaryotes, long stretches of DNA do not code for proteins or RNAs. Some of these non-coding regions include important regulatory sequences, but most have no known function. Because little or no selection pressure acts on them, they often change the fastest over evolutionary time. Protein-coding regions usually evolve more slowly. A change in a coding sequence can alter a protein and make it less effective at doing its job. That creates...

genome size and the evolution of new genes

Genome size can vary widely, and it also helps show how new genes form. Every living organism has a genome, whether it uses RNA or DNA. Yet the size of that genome can be very different from one organism to another. One major difference is whether the organism is prokaryotic or eukaryotic. Prokaryotic genomes usually have little to no non-coding sequence, which means genes are packed closely together in operons along the chromosome. Eukaryotic genomes contain long stretches of non-coding DNA...

gene families

How Gene Families Evolve and Diversify

Gene families are groups of genes that are thought to come from a shared ancestor. They usually form when a gene is copied by mistake during cell division. The original gene stays under selection pressure to keep its job, while the extra copy can change more quickly over time. Some copied genes gain a new role in the organism. When that happens, they are called paralogs, which are two genes in the same species that evolved from one common ancestral gene. Gene families also include orthologs,...

types of genetic transfer between organisms

types-of-genetic-transfer-between-organisms

How Genes Move Within and Across Generations

Genetic transfer is the movement of genetic information from one organism to another. It happens in two main ways: vertical gene transfer and horizontal gene transfer. Vertical gene transfer moves genes from one generation to the next. It is much more common than horizontal gene transfer. Vertical gene transfer happens during both sexual reproduction and asexual reproduction. In both cases, one or more organisms pass some or all of their genome to their offspring. This kind of transfer occurs...

key terms and concepts

Compare prokaryotic and eukaryotic genome sizes, then see how gene duplication, DNA shuffling, mutation, and horizontal gene transfer create new genes.

How Genome Size Shapes New Genes | JoVE K12

molecular biology

JoVE Core Biologia Molecular mostra os processos moleculares da célula através de vídeos de animação claros e concisos. Além disso, nos vídeos de Cientistas em ação, a aplicação desses conceitos é demonstrada por meio de experimentos reais em laboratórios modernos.

new-jersey-student-learning-standards-for-science

New Jersey Student Learning Standards for Science

High School

Life Science

heredity-inheritance-and-variation-of-traits

Heredity: Inheritance and Variation of Traits

HS-LS3-1

Florida Department of Education

SC.912.L.15

SC.912.L.15.2

Development of Antibiotic Resistance

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Understanding Species and Reproductive Barriers

A species is a group of organisms that interbreed and produce fertile offspring. Typically, individuals of the same species appear similar and share ...

Formation of Species

Speciation describes the formation of one or more new species from one or sometimes multiple original species. The resulting species are discrete from the ...

Speciation Rates

Speciation can proceed at markedly different rates, and evolutionary biologists commonly describe these differences through the models of gradualism and ...

Genetics of Speciation

Speciation is the evolutionary process resulting in the formation of new, distinct species&#x2014;groups of reproductively isolated populations.The ...

Hybrid Zones

Hybrid zones are narrow regions where two closely related species interact, mate, and produce hybrids. Relative to either parent species, hybrids may ...

What is Natural Selection?

Natural selection is an evolutionary process in which individuals with survival-promoting traits reproduce at higher rates. These favorable traits become ...

Types of Selection

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Frequency-dependent Selection

When the fitness of a trait is influenced by how common it is (i.e., its frequency) relative to different traits within a population, this is referred to ...

Limits to Natural Selection

Organisms that are well-adapted to their environment are more likely to survive and reproduce. However, natural selection does not lead to perfectly ...

Natural Selection

Fitness
Widespread variation of phenotypes in natural populations provides the raw material for evolution, which is the change in the inherited traits of ...

Simulating Natural Selection
ExpandTo prepare for the lab, set out workstations for each student pair containing 6 white, 6 black, and 13 gray 12 inch ...

Simulating Natural Selection
ExpandTo begin, cut the white, black, and gray pipe cleaners into quarters, which should produce a total of 100 pieces that ...

Artificial Selection

Natural Selection and Adaptive Evolution
In natural settings, adaptive evolution takes place by natural selection, a process in which differences in ...

Observing Artificial Selection in Plants
ExpandTwo weeks before beginning the pollination experiments, collect a sufficient amount of small pots with a ...

Observing Artificial Selection in Plants 
ExpandTo begin the experiment, collect five two-week old grown plants from the generation one population that ...

Analyze and interpret data to summarize how various factors such as geographic isolation, pesticide resistance, antibiotic resistance can influence natural selection.

LS.Bio.9.1

Phylogenetic Trees

Phylogenetic trees come in many forms. It matters in which sequence the organisms are arranged from the bottom to the top of the tree, but the branches ...

Conditions on Early Earth

Around 4 billion years ago, oceans began to condense on earth while volcanic eruptions released nitrogen, carbon dioxide, methane, ammonia, and hydrogen ...

The Colonization of Land

Changes in the environment of the early Earth drove the evolution of organisms. As prokaryotic organisms in the oceans began to photosynthesize, they ...

What is Evolutionary History?

Scientists record evolutionary history by analyzing fossil, morphological, and genetic data. The fossil record documents the history of life on Earth and ...

The Evidence for Evolution

Genetic variations accumulating within populations over generations give rise to biological evolution. Evolutionary changes can result in the formation of ...

The Fossil Record

The fossil record documents only a small fraction of all organisms that have ever inhabited Earth. Fossilization is a rare process, and most organisms ...

Convergent Evolution

Evolution shapes the features of organisms over time, ensuring that they are suited for the environments in which they live. Sometimes, selection pressure ...

Gene Evolution - Fast or Slow?

The genomes of eukaryotes are punctuated by long stretches of sequence which do not code for proteins or RNAs. Although some of these regions do contain ...

While every living organism has a genome of some kind (be it RNA, or DNA), there is considerable variation in the sizes of these blueprints. One major ...

Gene Families

Gene families consist of groups of genes proposed to have originated from a common ancestor. Typically these arise through events in which a gene or genes ...

Types of Genetic Transfer Between Organisms

Genetic transfer occurs when genetic information is passed from one organism to another. It occurs via two mechanisms: vertical gene transfer and ...

Ribozymes

The term ribozyme is used for RNA that can act as an enzyme. Ribozymes are mainly found in selected viruses, bacteria, plant organelles, and lower ...

Construct an explanation to illustrate how common ancestry and biological evolution are supported by multiple lines of empirical evidence.

Use models to illustrate the conditions required for natural selection, including the overproduction of offspring, inherited variation, and the struggle to survive.

LS.Bio.9.3

Construct an explanation to explain how natural selection leads to adaptations within populations.

LS.Bio.9.4

Extinction

The History of Life on Earth
Fossils of organisms that were different from anything alive today had been documented as early as the 17th century, but it ...

Diversity and Extinction during the Paleogene Epoch
ExpandFirst, familiarize yourself with the extinction reading supplement for the background on what ...

Diversity and Extinction during the Paleogene Epoch
ExpandBegin this procedure by reading the extinction reading supplement that has been printed out for ...

Construct explanations to illustrate how varying environmental conditions may result in: changes in the number of individuals of a species, the emergence of new species over time, or the extinction of other species.

LS.Bio.10.1

Synteny and Evolution

John H. Renwick first coined the term &#8220;synteny&#8221; in 1971, which refers to the genes present on the same chromosomes, even if they are not ...

Multi-species Conserved Sequences

Next-generation sequencing technologies have created large genomic databases of a variety of animals and plants. Ever since the human genome project was ...

Taxonomy

Taxonomy is the science of defining and naming groups of biological organisms based on shared characteristics. It uses a hierarchy of increasingly ...

Phylogeny

Phylogeny is concerned with the evolutionary diversification of organisms or groups of organisms. A group of organisms with a name is called a taxon ...

Use models (including dichotomous keys, scientific nomenclature, cladograms, phylogenetic trees) to identify organisms and exemplify relationships.

LS.Bio.10.2

Understand natural selection as a mechanism for biological evolution.

How Genome Size Shapes New Genes

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