Name: lipid anchors in membrane proteins
Uploaded: 2023-04-30T09:16:02.000Z
Duration: 1 min 32 s
Channel: JoVE

K12 Standards

Alabama Course of Study Science

9-12

Biology

from-molecules-to-organisms-structures-and-processes

From Molecules to Organisms: Structures and Processes

9-12.B.1.4.a

Lipids as Anchors

Os três tipos mais comuns de âncoras lipídicas são grupos prenil, grupos acila gordurosa e âncoras glicosilfosfatidilinositol ou GPI.
Os grupos prenil são os grupos farnesil de 15 carbonos e os grupos geranilgeranil de 20 carbonos. Eles se ligam a resíduos de cisteína no terminal carboxi das proteínas ou próximo a ele. 
O ácido mirístico de 14 carbonos saturado e o ácido palmítico de 16 carbonos são as âncoras de acila graxa mais comuns ligadas às proteínas.
A adição de ácido mirístico, ou miristoilação, ocorre no resíduo de glicina N-terminal de uma proteína. A palmitoilação das proteínas pode ocorrer no resíduo de cisteína N- ou C-terminal.
As proteínas preniladas, miristoiladas e palmitoiladas desempenham um papel essencial nas vias de sinalização intracelular e nas interações proteína-proteína.
As âncoras GPI se ligam a proteínas extracelulares e contêm uma estrutura central de fosfatidilinositol, glucosamina, três manonoses e fosfoetanolamina.
A fosfoetanolamina se liga ao aminoácido C-terminal da proteína alvo e o fosfolipídio se insere na camada externa da membrana.
Isso permite que as proteínas ancoradas em GPI participem de funções extracelulares, como comunicação célula-célula e adesão celular.

lipídios como âncoras

Na membrana plasmática, os lipídios que formam a bicamada também podem atuar como uma âncora para prender as proteínas à membrana. Os três principais tipos de âncoras lipídicas encontradas em eucariotos são: grupos prenil, grupos acil graxos e grupos glicosilfosfatidilinositol, ou GPI. Os grupos prenil e acil graxo atuam como âncoras na superfície citosólica da membrana, enquanto o GPI ancora proteínas no lado extracelular. O terminal carboxi da maioria das proteínas preniladas, como as proteínas Ras, contém o motivo CaaX, ou caixa CaaX, onde "C" significa cisteína, "a" significa qualquer aminoácido alifático e "X" significa qualquer outro resíduo aminoácido. O aminoácido presente na posição "X" determina se a ligação à proteína será a ligação farnesil ou geranilgeranil. Se o “X” for alanina, serina, metionina, cisteína ou glutamina, então a farnesil transferase reconhece o motivo CaaX e liga o farnesil à proteína. Por outro lado, se o "X" for ácido glutâmico ou leucina, então a geranil transferase tipo I identifica o motivo CaaX e liga o geranilgeranil a ele. Após a prenilação, o tripeptídio aaX é removido por proteases específicas e uma metiltransferase metila o resíduo de cisteína carregado negativamente resultante. A metilação remove a carga negativa presente na cisteína e a torna hidrofóbica. Às vezes, mais de uma âncora lipídica é usada para ligar a proteína à membrana. Por exemplo, a tirosina quinase citoplasmática é ancorada à membrana com a ajuda de ácido mirístico e palmítico. No entanto, as proteínas ancoradas por GPI geralmente não necessitam de outra âncora para estabilização. Uma extremidade do GPI contém os dois grupos acil graxos que podem ser inseridos na bicamada lipídica. Em contraste, na outra extremidade, a fosfoetanolamina liga-se às proteínas-alvo envolvidas em diferentes funções celulares.

Lipídios como âncoras

Na membrana plasmática, os lipídios que formam a bicamada também podem atuar como uma âncora para amarrar as proteínas à membrana. Os três principais tipos de âncoras lipídicas ...

Education

JoVE Core

Cell Biology

Biologia Celular

Unit 1

Unidade 1

Membrane Structure and Components

Estrutura e Componentes da Membrana

KEY TERMS AND CONCEPTS

TERMOS-CHAVE E CONCEITOS

lipid anchors in membrane proteins

Lipid anchors help attach proteins to the plasma membrane. In eukaryotic cells, the main lipid anchors are prenyl groups, fatty acyl groups, and glycosylphosphatidylinositol, or GPI, groups. Prenyl and fatty acyl groups anchor proteins on the cytosolic side of the membrane. GPI anchors proteins on the extracellular side.

Prenylated proteins often have a CaaX motif at the carboxy terminus. In this sequence, C means cysteine, a means any aliphatic amino acid, and X means any other amino acid residue. The amino acid at the X position helps decide the type of linkage. If X is alanine, serine, methionine, cysteine, or glutamine, farnesyl transferase attaches a farnesyl group. If X is glutamic acid or leucine, geranylgeranyl transferase type I attaches a geranylgeranyl group.

After prenylation, specific proteases remove the aaX tripeptide. Then a methyltransferase methylates the cysteine that remains. This step removes the negative charge on the cysteine and makes it more hydrophobic. The change helps the protein stay associated with the membrane.

Some proteins use more than one lipid anchor. A cytoplasmic tyrosine kinase, for example, is anchored with both myristic acid and palmitic acid. GPI-anchored proteins usually do not need a second anchor for stability. The GPI group has two fatty acyl groups that insert into the lipid bilayer. Its other end contains phosphoethanolamine, which links to target proteins involved in different cell functions.

Lipid Anchors in Membrane Proteins

what are membranes?

Membrane Receptors and Cell Signals

The plasma membrane sets the outer boundary of a cell and helps control how the cell interacts with its environment. It lets some substances enter, keeps others out, and releases some materials in controlled amounts. The membrane also needs to stay flexible so cells such as red and white blood cells can change shape as they move through narrow capillaries. The membrane surface also carries markers that help cells recognize one another. This recognition is important during early development,...

membrane fluidity

How Cell Membranes Stay Flexible

Cell membranes stay flexible because of the fluid mosaic model. The plasma membrane is a mosaic of phospholipids, cholesterol, proteins, and carbohydrates. These parts are separate but loosely attached, so the membrane keeps a fluid character. The membrane is still fairly rigid, but it can flow and self-seal after a very fine needle passes through it. Phospholipid tails also affect membrane fluidity. Saturated fatty acids in phospholipids have straight tails because they are packed with...

fluid mosaic model

Fluid Mosaic Model of the Membrane

The fluid mosaic model explains the structure and function of the plasma membrane. Scientists first identified the plasma membrane in the 1890s, and by 1915 they had identified its main chemical parts: lipids and proteins. Early electron micrographs showed a railroad track pattern, which helped lead to the first widely accepted membrane model in 1935.

membrane lipids

Membrane Lipids in Cells and Tissues

Membrane lipids give biological membranes their structure and help set their properties. In mammalian cells, lipids make up about 50% of the membrane on average. The amount can be much lower in the inner mitochondrial membrane or much higher in the myelin sheath around nerve cells. Several phospholipids are common in mammalian membranes. These include phosphatidylcholine, phosphatidylethanolamine, phosphatidylserine, and sphingomyelin. At physiological pH, phosphatidylserine carries a negative...

asymmetric lipid bilayer

Membrane Lipid Unevenness

Biological membranes have an uneven mix of lipids between the inner and outer layers. This membrane asymmetry gives each side a different chemical makeup and supports different cell functions. Evidence for this uneven layout came from treating an erythrocyte membrane with phospholipase. Phospholipase is an enzyme that breaks lipids into fatty acids and hydrophilic groups. It acted only on the outer layer, while the inner layer stayed intact. The treatment degraded about 80% of total membrane...

membrane asymmetry regulating transporters

membrane-asymmetry-regulating-transporters

Membrane Lipid Flipping and Transport

Membrane lipid flipping and transport help cells keep an uneven, or asymmetric, distribution of phospholipids across the membrane. This balance is controlled by flippase, floppase, and scramblase enzymes, which move lipids between the two membrane leaflets. Flippases move specific phospholipids from the outer leaflet to the inner leaflet. In eukaryotes, these enzymes are type-IV P-type ATPases, also called P4-ATPases. They are membrane pumps that use ATP and contain one transmembrane domain...

membrane carbohydrates

Membrane Sugars and Cell Identity

Membrane carbohydrates help give cells their surface identity and support plasma membrane function. The plasma membrane is a dynamic barrier made of lipids, proteins, and carbohydrates. These sugar chains have unique structural and chemical traits that help the membrane work properly for cell growth and survival. Membrane carbohydrates do not have a hydrophobic region, so they are found only on the outer surface of the cell. Sugar addition, or glycosylation, of proteins occurs in the lumens of...

membrane proteins

Facilitated Transport Across Cell Membranes

Facilitated transport uses membrane proteins to help materials cross the plasma membrane. These integral transmembrane proteins act as channels or carriers. They move selected substances through the membrane more easily than the lipid layer alone would allow. Channel proteins provide a hydrophilic, or water-friendly, path through the membrane. Their core forms a hydrated opening that lets solutes pass between the inside and outside fluids of the cell. This helps polar compounds avoid the...

lipids as anchors

Lipid anchors help attach proteins to the plasma membrane. In eukaryotic cells, the main lipid anchors are prenyl groups, fatty acyl groups, and glycosylphosphatidylinositol, or GPI, groups. Prenyl and fatty acyl groups anchor proteins on the cytosolic side of the membrane. GPI anchors proteins on the extracellular side. Prenylated proteins often have a CaaX motif at the carboxy terminus. In this sequence, C means cysteine, a means any aliphatic amino acid, and X means any other amino acid...

single-pass transmembrane proteins

How Membrane Proteins Span Cell Membranes

Membrane proteins help cells communicate, send signals, stick to other cells, and move molecules across the membrane. Integral membrane proteins are tightly linked to the cell membrane and carry out these jobs. Some integral membrane proteins sit only in one membrane layer. Fatty acid amide hydrolase is one example, and it is found on the cytoplasmic side of the membrane monolayer. Other integral membrane proteins are transmembrane proteins, which span across the membrane. These proteins have...

multi-pass transmembrane proteins and &#946;-barrels

multi-pass-transmembrane-proteins-and-barrels

Membrane-Spanning Helices and β-Barrels

Membrane-spanning proteins can cross the cell membrane more than once. Their transmembrane chain usually folds into either an alpha-helix or a beta-strand. Alpha-helix multi-pass proteins are common, while beta-strand proteins are found mainly in gram-negative bacteria, mitochondria, and chloroplasts. Some multi-pass alpha-helix proteins are G-protein-linked receptors, or GPCRs, and bacteriorhodopsin. GPCRs have seven transmembrane alpha-helices. Each receptor also has its own extracellular...

detergent purification of membrane proteins

detergent-purification-of-membrane-proteins

How Detergents Solubilize Membrane Proteins

Detergents help solubilize membrane proteins so they can be purified from the lipid bilayer. Their hydrophobic region can replace membrane phospholipids and keep integral membrane proteins in solution. A detergent works well only when its monomers reach the critical micelle concentration, or CMC. At that point, the molecules assemble into micelles, which are small clusters of detergent. Above the CMC, free detergent monomers stay in equilibrium with the micelles. The CMC varies with the...

protein diffusion in the membrane

How Membrane Proteins Move Side to Side

Membrane proteins can move across the cell surface by lateral diffusion, and they can also rotate in place. Scientists confirmed lateral movement with a cell fusion experiment. Mouse and human cells were fused to make hybrid cells. The membrane proteins on the two cells were tagged with red and green fluorescent markers. At first, each color stayed on its own side of the fused cell. After 40 minutes, the colors mixed across the whole cell surface, showing that the proteins had moved laterally. <br...

membrane domains

Lipid Rafts and Sperm Membrane Regions

Membrane domains organize specific lipids and proteins in defined parts of a cell membrane. This arrangement supports cell signaling, adhesion, and other important cellular processes. These domains can vary in size, composition, function, and how long they last. Some membrane domains are built from proteins. The membrane can contain distinct protein groups that carry out a cell’s specific functions. A clear example is the plasma membrane of a human sperm cell, which has different proteins in...

mechanisms of membrane domain formation

How Cell Membranes Form Domains

Cell membranes form domains when lipids and proteins gather into distinct regions. These regions, or membrane domains, are shaped by different physical properties that help some molecules stay together while others move apart. Some domains form through protein-protein interactions. Others depend on specific lipids, such as sphingolipids and sterols. Large proteins, including bacteriorhodopsin, can also cluster and create visible membrane domains. Membrane proteins can be held in place by...

mechanisms of membrane-bending

How Proteins Shape Cell Membranes

Cell membranes can bend into many shapes because they are flexible and made of a fluid mosaic. That bending can be temporary, as in vesicles, or long-lasting, as in microvilli. Cells control the size, place, and time of membrane curvature. Membrane bending can start inside the membrane itself or from proteins attaching to it. Changes in lipid makeup can create curvature, and proteins can help by making the membrane asymmetric or by clustering certain lipids. The shape of integral proteins also...

key terms and concepts

Learn how prenyl, fatty acyl, and GPI anchors attach proteins to the plasma membrane, including the CaaX motif and key enzyme steps.

Lipid Anchors in Membrane Proteins | JoVE K12

cell biology

JoVE Core - Biologia Celular ilustra processos celulares complexos em vídeos de animação fáceis de entender e, ao mesmo tempo, abrangentes. Esta coleção foca nos componentes e funções essenciais das células, incluindo organelas e membranas, transporte celular e energia celular. É complementado por vídeos de Cientistas em ação que demonstram a aplicação da biologia celular em experimentos de pesquisa do mundo real.

Florida Department of Education

High School

Life Science

SC.912.L.18

SC.912.L.18.3

What are Cells?

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Cytoplasm

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The Nucleus

The nucleus is a membrane-bound organelle that acts as a control center in a eukaryotic cell. It contains chromosomal DNA, which controls gene expression ...

Endoplasmic Reticulum

Endoplasmic ReticulumThe endoplasmic reticulum (ER) is an extensive network of membranous sacs and tubules in eukaryotic cells, continuous with the outer ...

Ribosomes

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Golgi Apparatus

As they leave the Endoplasmic Reticulum (ER), properly folded and assembled proteins are selectively packaged into vesicles. These vesicles are ...

Lysosomes

Lysosomes are membrane-enclosed spherical sacs derived from the Golgi apparatus. The most important function of the lysosome is degrading macromolecules ...

Peroxisomes

Peroxisomes and mitochondria are two important oxygen-utilizing organelles in eukaryotic cells. Mitochondria carry out cellular respiration&#x2014;the ...

Introduction to the Cytoskeleton

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Introduction to Actin

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overview-of-myosin-structure-and-function

Overview of Myosin Structure and Function

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Microtubules

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The Structure of Intermediate Filaments

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Types of Intermediate Filaments

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Gap Junctions

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The Extracellular Matrix

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Tissues

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Plant Cell Wall

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pH Regulation in Cells

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Chemistry of the Cell

Introduction to Cellular Respiration

Organisms harvest energy from food, but this energy cannot be directly used by cells. Cells convert the energy stored in nutrients into a more usable ...

ATP Energy Storage and Release

ATP and Macromolecule Synthesis

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1. Engage in evidence-based argument to relate a cell's function to the structure, function, and diversity of its components. Examples: Muscle cells have a large number of mitochondria, which create energy for contraction in the form of ATP. Some plant cells have a large vacuole which is used for storage and transport of excess nutrients in changing environments.

9-12.B.1.1

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RNA Structure

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Types of RNA

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Transcription

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Eukaryotic RNA Polymerases

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RNA Polymerase II Accessory Proteins

Transcription Elongation Factors

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Pre-mRNA Processing: Modification of pre-mRNA Ends

In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or ...

Pre-mRNA Processing: RNA Splicing

Chromatin Structure and RNA Splicing

Alternative RNA Splicing

Nuclear Export of mRNA

Transfer RNA Synthesis

Ribosomal RNA Synthesis

The Nucleolus

Additional Subnuclear Structures

Translation

Translation is the process of synthesizing proteins from the genetic information carried by messenger RNA (mRNA). Following transcription, it constitutes ...

tRNA Activation

Initiation of Translation

Termination of Translation

Improving Translational Accuracy

prokaryotic-transcriptional-activators-and-repressors

Prokaryotic Transcriptional Activators and Repressors

Nonsense-mediated mRNA Decay

The Eukaryotic Promoter Region

Molecular Chaperones and Protein Folding

Co-activators and Co-repressors

The Proteasome

Master Transcription Regulators

Regulated Protein Degradation

Regulated mRNA Transport

Proteins: From Genes to Degradation

mRNA Stability and Gene Expression

Cell Specific Gene Expression

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between ...

regulation-of-expression-occurs-at-multiple-steps

Regulation of Expression Occurs at Multiple Steps

Small interfering RNAs (siRNA)

Cis-regulatory Sequences

lncRNA - Long Non-coding RNAs

cooperative-binding-of-transcription-regulators

Cooperative Binding of Transcription Regulators

Epigenetic Regulation

Epigenetic changes alter the physical structure of the DNA without changing the genetic sequence and often regulate whether genes are turned on or off. ...

2. Obtain and evaluate information to explain the role of DNA and RNA in transcription and translation leading to protein synthesis and cellular function.

9-12.B.1.2

Nucleic Acids and Nucleotides

Nucleic acids are the most important macromolecules for the continuity of life. They carry the cell&#39;s genetic blueprint and have instructions for its ...

Nucleic Acid Structure

The pentose sugar in DNA is deoxyribose, while in RNA the pentose sugar is ribose. The difference between the sugars is the presence of the hydroxyl group ...

a. Use a model to describe the structure and sequence of DNA, including nucleotide structure, base pairing, and the structure of the helix.

9-12.B.1.2.a

Lipid Anchors in Membrane Proteins

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