18.1
Endocitose
As células eucarióticas adquirem nutrientes para crescimento e proliferação. Nutrientes e outras moléculas que requerem degradação são internalizados…
As células eucarióticas ingerem moléculas do espaço extracelular por um processo chamado endocitose.
Na endocitose, uma parte da membrana plasmática se dobra para dentro para envolver uma molécula que chega e, em seguida, brota para formar uma vesícula de transporte.
A vesícula de transporte transporta as moléculas para o citosol para fusão com outras organelas intracelulares, como endossomos ou lisossomos. Após a fusão com o lisossomo, o material ingerido pode ser degradado com a ajuda de enzimas lisossômicas. Por outro lado, algumas moléculas endocitadas devem ser liberadas na célula para utilização.
A endocitose permite que as células absorvam nutrientes, certas proteínas e uma variedade de moléculas solúveis.
Algumas células especializadas, como fagócitos, também engolfam detritos celulares, partículas estranhas ou organismos, como bactérias ou protozoários, como parte do mecanismo de defesa do hospedeiro. Esse tipo de endocitose é conhecido como fagocitose.
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Q1: How does endocytosis allow cells to bring molecules into the cytosol?
In endocytosis, the plasma membrane bends inward to surround an incoming molecule, then buds off to form a transport vesicle. This vesicle carries molecules into the cytosol for fusion with intracellular organelles like endosomes or lysosomes. The process enables cells to uptake nutrients, proteins, and soluble molecules from the extracellular space.
Q2: What happens to endocytosed material after it fuses with a lysosome?
Once endocytosed material fuses with a lysosome, lysosomal enzymes degrade the ingested material. However, some endocytosed molecules must be released in the cell for utilization rather than degradation. This sorting determines whether material is broken down or used by the cell for growth and proliferation.
Q3: What is phagocytosis and what role does it play in host defense?
Phagocytosis is a specialized type of endocytosis where phagocytes engulf pathogenic microorganisms, parasites, and cell debris. The internalized pathogens are contained in a vesicle called a phagosome, which fuses with a lysosome for degradation. This process is vital for providing immunity to the host organism.
Q4: How does receptor-mediated endocytosis differ from other endocytic pathways?
Receptor-mediated endocytosis is a highly specific process where an incoming molecule is internalized only after binding to its associated receptor. The coated vesicle containing the endocytosed molecule fuses with the early endosome. This selectivity allows cells to regulate which molecules enter through targeted receptor recognition.
Q5: What is pinocytosis and how does it differ from phagocytosis?
Pinocytosis is endocytosis where cells engulf dissolved substances from the extracellular space, unlike phagocytosis which targets large particles and pathogens. In pinocytosis, a coated vesicle containing the endocytosed fluid fuses with an early endosome. This process allows cells to internalize small soluble molecules for nutrient acquisition.
Q6: What role do clathrin-coated vesicles play in endocytosis?
Transport vesicles formed during endocytosis can be coated with a protein called clathrin. These clathrin-coated vesicles protect and transport endocytosed material as they bud from the plasma membrane. The coating helps organize and facilitate the movement of molecules into the cytosol for subsequent sorting and processing.
Q7: How can pathogenic organisms exploit endocytosis to infect host cells?
Some pathogenic organisms take advantage of endocytosis to enter cells and establish infection. By mimicking or binding to cellular receptors, these pathogens trigger endocytic uptake into the host cell. Once internalized, they can evade immune defenses or manipulate cellular machinery for replication and survival.