Analisando a função de GTPases Pequenas pela microinjeção de Plasmids em Polarized Células Epiteliais
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Summary
Abstract
Protocol
Discussion
Disclosures
The authors have nothing to disclose.
Acknowledgements
Materials
| Axiovert 200 Microscope with heated stage | Carl Zeiss, Inc. | Custom order | |
| Injectman NI2 Femtojet micromanipulator | Eppendorf | Custom order | |
| Femtotips II (Microinjection needles) | Eppendorf | 930000043 | |
| Microloader tips | Eppendorf | 930001007 | |
| Clear 12-mm transwell filter supports | Corning | 3460 | |
| Endotoxin-free plasmid maxiprep kit | Sigma-Aldrich | NA0400 |
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Cite This Article
Cook, R. N., Ang, S. F., Kang, R. S., Fölsch, H. Analyzing the Function of Small GTPases by Microinjection of Plasmids into Polarized Epithelial Cells. J. Vis. Exp. (51), e2645, doi:10.3791/2645 (2011).