É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.
Capítulos
- 00:00Título
- 00:47Introduction
- 01:23Assembly of Construct Casting Molds
- 03:25Preparation for Myoblast Cell Isolation
- 04:56Myoblast Isolation from Skeletal Muscle
- 09:09Casting Engineered Tissue Constructs
- 13:17Conclusion
Here, we demonstrate fabrication of collagen-based, tissue constructs containing skeletal myoblasts. These 3-D engineered constructs may be used to replace or repair tissues in vivo. For our purposes, we have designed these as an atrioventricular electrical conduit for the repair of complete heart block[1].
Tags
FabricationElectronic PacemakersPediatric PatientsCardiac Rhythm DisordersCollagen-based MatrixAutologously-derived CellsRecurrent SurgeriesQuality Of LifePrimary Skeletal Myoblast Cell CulturesHydrogel MatrixElectrical ConduitUpper And Lower Chambers Of The HeartAtrioventricular Electrical ConductionComplete Heart BlockMyoblastsSkeletal MusclesNeonatal Lewis RatsAntibioticsType 1 CollagenMatrigel™NaHCO3
