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Construct Design for Transfecting Cysteine-Engineered Voltage-Gated Ca2+ Channels in Murine Isolated Skeletal Muscle Fibers (Video) | JoVE

Functional Site-Directed Fluorometry in Native Skeletal Muscle Cells

Article DOI: 10.3791/200065-v • 01:22 min • June 2nd, 2023
June 2nd, 2023 •
1, 1, 1, 1
1Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine

Tags

Functional Site-Directed Fluorometry Native Cells Skeletal Muscle Excitability CaV1.1 Voltage-gated Ion Channels Voltage Sensors Ryanodine Receptor Activation Calcium Current Excitation-contraction Coupling Calcium Channel Activation Cyro-electron Microscopic Structure CaV1.1 Accessory Protein Channel Alternative Splicing Variant Disease-causing Mutation Electrophysiology Molecular Biology Cryo-electron Microscopy Molecular Dynamic Simulation Targeted Protein Degradation Engineered Cells Animal Models Protein-protein Interactions
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