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Autophagy Protease Tag-Compound Addition and Cellular Supernatant Harvest for Drug Screening (Video) | JoVE

High-Throughput Screening Analysis for 4B Cysteine Peptidase Autophagy Inhibitor

Article DOI: 10.3791/200236-v • 01:48 min • June 30th, 2023
June 30th, 2023 •
1, 1, 1,2
1Laboratory for Molecular Cell Biology, University College London, 2Department of Human Medicine, Medical School Berlin

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Cell-based Drug Screening Autophagy ATG4 Cysteine Peptidase Small Molecule Inhibitors High Content Screening High Throughput Screening Drug Targets Drug Candidates Pancreatic Cancer ATG4 Cysteine Protease Family Dysfunction Post-translational Mechanisms Activators Assays Luciferase-based Assay Protease Activity Knockout Cell Lines
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