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A Standardized Method for Detecting AAV Vectors in Human Tears Using ddPCR (Video) | JoVE
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A Standardized Method for Detecting AAV Vectors in Human Tears Using ddPCR (Video) | JoVE

Quantification of Viral Vectors in Tears Using ddPCR for Bioshedding Studies

Article DOI: 10.3791/200301-v • 05:50 min • July 14th, 2023
July 14th, 2023 •
1, 1, 1, 1
1Cell and Gene Therapy, KCAS Bioanalytical and Biomarker Services

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Validatable Droplet Digital Polymerase Chain Reaction (ddPCR) Assay Detection Adeno-associated Viral Vectors Bioshedding Studies Tears Quantification Clinical Trials Regulatory Gaps Good Laboratory Practices Molecular Studies Viral Vector Development Regulatory Agencies Bio-shedding Assessment Standard Curve Sensitivity Complex Matrices PCR Interference Inhibition Clinical Sample Amounts Rare Events Detection Assay Validation Repeatable Framework Adaptable Framework Streamlined Validation Criteria For Assay Performance Evaluation
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