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Sample Preparation, RNA Extraction, PCR Clean Up, and Quantification for Whole Genome Sequencing of Rabies Virus (RABV) (Video) | JoVE

A Nanopore-Based Genomic Approach to Control Rabies in Low-Income Countries

Article DOI: 10.3791/200317-v • 03:08 min • August 18th, 2023
August 18th, 2023 •
1,2, 1,3,4,5, 1,6, 1, 1, 1,6, 7,8, 3,9, 1, 1,6
1School of Biodiversity, One Health & Veterinary Medicine, University of Glasgow, 2Research Institute for Tropical Medicine, 3University of Nairobi, Institute of Tropical and Infectious Diseases, 4Tanzania Industrial Research Development Organization, 5Ifakara Health Institute, 6MRC-University of Glasgow Centre for Virus Research, 7Department of Veterinary Public health, Ahmadu Bello University, 8African Centre of Excellence for Neglected Tropical Diseases and Forensic Biotechnology, Ahmadu Bello University, 9University of Nairobi, Kenya and Center for Epidemiological Modelling and Analysis, University of Nairobi

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Séquençage du génome entier caractérisation rapide virus de la rage technologie des nanopores données génomiques suivi de la transmission propagation géographique maladies infectieuses pays à revenu faible et intermédiaire (PRFI) rage transmise par les chiens chauves-souris vampires santé publique préoccupations économiques flux de travail de l’échantillon à la séquence et à l’interprétation technologie des nanopores collecte d’échantillons diagnostic de la rage flux de travail de séquençage du génome entier conception et optimisation des amorces réaction en chaîne par polymérase multiplex (PCR) séquençage Préparation de la bibliothèque Appel de base en direct et hors ligne Désignation de lignées génétiques Analyse phylogénétique Mise en œuvre de flux de travail Validation du pipeline Contrôles négatifs Outils génomiques (GLUE MADDOG) Phylogénies régionales et mondiales
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