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JoVE Journal Biochemistry

MHCI and MHCII Analysis of Single Fibers Isolated from Freeze-Dried Muscle Samples Using Dot Blot (Video) | JoVE

Improved UPLC-UV Method for the Quantification of Vitamin C in Lettuce Varieties (Lactuca sativa L.) and Crop Wild Relatives (Lactuca spp.)

Journal (Biochemistry)

Improved UPLC-UV Method for the Quantification of Vitamin C in Lettuce Varieties (Lactuca sativa L.) and Crop Wild Relatives (Lactuca spp.)

Ganglioside Extraction, Purification and Profiling

Journal (Biochemistry)

Ganglioside Extraction, Purification and Profiling

MHCI and MHCII Analysis of Single Fibers Isolated from Freeze-Dried Muscle Samples Using Dot Blot (Video) | JoVE

MyDoBID- A Novel Technique for Identifying Muscle Fiber Types Efficiently

Article DOI: 10.3791/200550-v • 04:08 min • September 22nd, 2023

September 22nd, 2023 •

Heidy K. Latchman*¹, Stefan G. Wette*¹, Dion J. Ellul¹, Robyn M. Murphy¹, Noni T. Frankenberg¹

¹Department of Biochemistry and Chemistry, La Trobe Institute for Molecular Science, School of Agriculture, Biomedicine and Environment, La Trobe University

* These authors contributed equally

Tags

Fiber Type Identification Skeletal Muscle Myosin Heavy Chain Dot Blotting MyDoBID Muscle Fiber Type Classification MHCI-antibodies IIa-specific Antibodies Biopsy Samples Sodium Dodecyl-sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE) UV-activated Gel Technology Western Blotting Protein Loading Normalization Single-fiber Western Blots Sample Versatility Sample Throughput Time Investment Cost-saving Measures

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