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A 96 Well Microtiter Plate-based Method for Monitoring Formation and Antifungal Susceptibility Testing of Candida albicans Biofilms

Article DOI: 10.3791/2287-v • 07:20 min • October 21st, 2010
October 21st, 2010 •
1,2, 1,2, 1,2, 1,2
1Department of Biology, University of Texas San Antonio - UTSA, 2South Texas Center for Emerging Infectious Diseases, University of Texas San Antonio - UTSA

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We describe a simple, rapid and robust method for the formation of Candida albicans biofilms using 96 well microtiter plates and its utility in antifungal susceptibility testing of cells within biofilms.

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96 Well Microtiter Plate Monitoring Formation Antifungal Susceptibility Testing Candida Albicans Biofilms Candidiasis Fungal Infections Host Tissues Medical Devices Catheters In Vitro Model Colorimetric Assay XTT Tetrazolium Salt Metabolically Active Fungal Biofilm Cells Experiment Duration Antifungal Susceptibility Testing Standardization
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