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ReAsH/FlAsH Labeling and Image Analysis of Tetracysteine Sensor Proteins in Cells
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ReAsH/FlAsH Labeling and Image Analysis of Tetracysteine Sensor Proteins in Cells

Article DOI: 10.3791/2857-v • 12:20 min • August 31st, 2011
August 31st, 2011 •
1, 1, 1, 1
1Department of Biochemistry and Molecular Biology, Bio21 Molecular Science and Biotechnology Institute

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The biarsenical dyes FlAsH and ReAsH bind specifically to tetracysteine motifs in proteins and can selectively label proteins in live cells. Recently this labeling strategy has been used to develop sensors for different protein conformations or oligomeric states. We describe the labeling approach and methods to quantitatively analyze binding.

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ReAsH FlAsH Labeling Image Analysis Tetracysteine Sensor Proteins Cells Fluorescent Proteins Protein Trafficking Protein Localization Protein Function Fusion Partners Conformational Change Protein-association Small Tag System Biarsenical Dyes Specificity Live Cells Steric Constraints Host Protein Conformational Change Measurement Protein-protein Interactions Oligomerization Sensors Mutant Huntingtin Aggregates Neurons Huntington Disease
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