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Isolation and Culture of Neural Crest Stem Cells from Human Hair Follicles
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Neurociência
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JoVE Journal Neurociência
Isolation and Culture of Neural Crest Stem Cells from Human Hair Follicles

Isolation and Culture of Neural Crest Stem Cells from Human Hair Follicles

DOI:
10.3791/3194-v

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07:00 min

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April 06, 2013

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1Department of Pathology and Laboratory Medicine,School of Medicine, University of Pennsylvania

Capítulos

  • 00:05Título
  • 01:12Preparation of Tissue Culture Plates and Media
  • 02:08Extract Hair Follicle Cells from Human Scalp
  • 03:37Isolation and Culture of Hair Follicle NCSCs Using Flow Cytometric Cell Sorting
  • 05:44Representative Hair Follicle Stem Cell Culturing Results
  • 06:42Conclusion

Summary

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Tadução automática

This article presents a robust protocol for isolation and culture of neural crest stem cells from human hair follicles.

Tags

Neural Crest Stem CellsHuman Hair FolliclesStem Cell ProliferationHair CycleHair BulgeOuter Root SheathEpithelial Stem CellsMelanocyte Stem CellsNeural Crest Like Stem CellsLabel-retaining CellsSelf-renewalAsymmetric Cell DivisionGene Expression PatternMyogenic Cell LineageMelanocytic Cell LineageNeuronal Cell LineageMesenchymal LineagesDifferentiation PotentialMouse Brain

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