Waiting
Processando Login

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Immunology and Infection / Determining Optimal Cytotoxic Activity of Human…
JoVE Journal
Immunology and Infection

Este de .

JoVE Journal Immunology and Infection
Determining Optimal Cytotoxic Activity of Human Her2neu Specific CD8 T cells by Comparing the Cr51 Release Assay to the xCELLigence System
Neutrophil Phagocytosis Assay
Journal (Immunology and Infection)
Neutrophil Phagocytosis Assay
A Semi-automated Approach to Preparing Antibody Cocktails for Immunophenotypic Analysis of Human Peripheral Blood
Journal (Immunology and Infection)
A Semi-automated Approach to Preparing Antibody Cocktails for Immunophenotypic Analysis of Human Peripheral Blood
 

Determining Optimal Cytotoxic Activity of Human Her2neu Specific CD8 T cells by Comparing the Cr51 Release Assay to the xCELLigence System

Article DOI: 10.3791/3683-v • 11:31 min • August 8th, 2012
August 8th, 2012 •
1, 1, 1
1Department of Immunology, College of Medicine, Mayo Clinic

Capítulos

Resumo
Automatic Translation

The chromium release assay, a common assay for detecting cytotoxic T cell activity, has several limitations. Using antigen-specific CD8 T cells and the human breast cancer tumor line, SKBR3, in the present article, an impedance-based approach was examined for the capability of detecting cell killing.

Tags

Cytotoxic Activity CD8 T Cells Cr51 Release Assay XCELLigence System T Cell Receptor (TCR) Antigen-specific CD8 T Cells ELIspot Flow Cytometry Cytotoxicity Assay Chromium Release Assay (CRA) Gamma Radiation Reproducibility Caspase Release BLT Esterase Activity CD107 Expression Impedance-based Assay Roche XCelligence System
Read Article
Waiting X
Simple Hit Counter