Journal
/
Neurociência
/
In ovo Electroporation in Chick Midbrain for Studying Gene Function in Dopaminergic Neuron Development
JoVE Journal
Neurociência
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Neurociência
In ovo Electroporation in Chick Midbrain for Studying Gene Function in Dopaminergic Neuron Development

In ovo Electroporation in Chick Midbrain for Studying Gene Function in Dopaminergic Neuron Development

DOI:
10.3791/4017-v

•

08:57 min

•

August 03, 2012

•

, ,
1Northwestern University Feinberg School of Medicine,Children’s Hospital of Chicago Research Center, 2Departments of Pediatrics, Neurology and Physiology,Northwestern University Feinberg School of Medicine

Capítulos

  • 00:05Título
  • 01:34Egg Preparation
  • 02:42Glass Injection Needle Preparation
  • 03:25Midbrain Injection
  • 04:38Electroporation
  • 05:39Incubate and Harvest
  • 06:47Results: Embryonic Chick Midbrain In ovo Electroporation Assay
  • 08:13Conclusion

Summary

Tadução automática

Tadução automática

To assess the function and the regulation of genes during the development of midbrain dopaminergic neurons, we describe a method that involves in ovo electroporation of plasmid DNA constructs into embryonic chick ventral midbrain dopaminergic neuron progenitors. This technique can be used to achieve efficient expression of genes of interest to study different aspects of midbrain development and dopaminergic neuron differentiation.

Tags

Ovo ElectroporationChick MidbrainGene FunctionDopaminergic Neuron DevelopmentParkinson’s DiseaseSchizophreniaDepressionDementiaNeural ProgenitorsGene Expression ProgramsExperimental ManipulationsChick Embryonic Neural TubesPCAG VectorCMV Promoter

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

In utero Electroporation followed by Primary Neuronal Culture for Studying Gene Function in Subset of Cortical Neurons

Transfection of Mouse Retinal Ganglion Cells by in vivo Electroporation

Mouse in Utero Electroporation: Controlled Spatiotemporal Gene Transfection

Organotypic Slice Cultures of Embryonic Ventral Midbrain: A System to Study Dopaminergic Neuronal Development in vitro

Dual Electrophysiological Recordings of Synaptically-evoked Astroglial and Neuronal Responses in Acute Hippocampal Slices

In ovo Expression of MicroRNA in Ventral Chick Midbrain

Directed Dopaminergic Neuron Differentiation from Human Pluripotent Stem Cells

In Vivo Calcium Imaging of Lateral-line Hair Cells in Larval Zebrafish

Studying Brain Function in Children Using Magnetoencephalography

3D Kinematic Analysis for the Functional Evaluation in the Rat Model of Sciatic Nerve Crush Injury

Read Article