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Optogenetic Activation of Zebrafish Somatosensory Neurons using ChEF-tdTomato

Article DOI: 10.3791/50184-v • 07:25 min • January 31st, 2013
January 31st, 2013 •
1, 1
1Department of Molecular, Cell, and Developmental Biology, University of California, Los Angeles

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Optogenetic techniques have made it possible to study the contribution of specific neurons to behavior. We describe a method in larval zebrafish for activating single somatosensory neurons expressing a channelrhodopsin variant (ChEF) with a diode-pumped solid state (DPSS) laser and recording the elicited behaviors with a high-speed video camera.

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Optogenetic Activation Zebrafish Somatosensory Neurons ChEF-tdTomato Neural Circuits Optogenetic Approaches Light-sensitive Ion Channels Larval Zebrafish Behavior Assessment Channelrhodopsin Variant Single Cell Activation Behavioral Responses Transgene Expression Confocal Microscopy DPSS Laser
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